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415049

Sigma-Aldrich

Aniline Blue diammonium salt

certified by the Biological Stain Commission

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Synonym(s):
Ink Blue R; 4-[4-[[4-(4-aminooxysulfonylanilino)phenyl]-[4-(4-aminooxysulfonylphenyl)iminocyclohexa-2,5-dien-1-ylidene]methyl]anilino]benzenesulfonic acid
Empirical Formula (Hill Notation):
C37H32N5O9S3
CAS Number:
66687-07-8
Molecular Weight:
786.87
UNSPSC Code:
12171500
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

200

form

powder, crystals or granules

composition

Dye content, 65%

color

brown to very dark brown, and Red to Very Dark Red and Red-Brown and Very Dark Red-Brown

ε (extinction coefficient)

15000 at 595-605 nm in water at 0.01 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

InChI

1S/C37H31N5O9S3/c38-50-53(46,47)35-21-15-32(16-22-35)41-29-9-3-26(4-10-29)37(25-1-7-28(8-2-25)40-31-13-19-34(20-14-31)52(43,44)45)27-5-11-30(12-6-27)42-33-17-23-36(24-18-33)54(48,49)51-39/h1-24,40-41H,38-39H2,(H,43,44,45)

InChI key

CSYADFXDZYPANK-UHFFFAOYSA-N

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General description

Aniline Blue is the traditional name applied to anionic dyes produced by successive phenylation and sulfonation of basic fuchsine. It is a mixture of methyl blue (Cl 42780, Acid blue 93) and water blue (Cl 42755, Acid blue 22).

Application

Aniline blue (nominally as methyl blue or water blue) is a component of commonly used polychrome stains. Heidenhain′s AZAN stain and the Mallory′s polychrome stain both entail staining steps using aniline blue-orange G mixtures. Aniline blue is used in Gomori′s one-step trichrome stain and Mallory′s connective tissue stain for tissue including kidney and intestine.

Suitability

Certified for use in Mallory′s connective tissue stain and Gomori′s one-step trichrome.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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