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I9889

Sigma-Aldrich

Anti-Human IgA (α-chain specific) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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MDL number:
NACRES:
NA.46

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

: 2.5-5.0 mg/mL using Antibody concentration by precipitin Vs Human serum
: 4.0-6.0 mg/mL using Antibody concentration by precipitin Vs Human whey
Ouchterlony double diffusion: 1:32

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
I9764B1395R9255
antibody form

IgG fraction of antiserum

antibody form

IgG fraction of antiserum

antibody form

IgG fraction of antiserum

antibody form

IgG fraction of antiserum

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

technique(s)

: 2.5-5.0 mg/mL using Antibody concentration by precipitin Vs Human serum, Ouchterlony double diffusion: 1:32, : 4.0-6.0 mg/mL using Antibody concentration by precipitin Vs Human whey

technique(s)

immunoelectrophoresis: suitable, indirect ELISA: 1:50,000, quantitative precipitin assay: 2.0-3.0 mg/mL

technique(s)

indirect ELISA: 1:150,000, quantitative precipitin assay: 2.0 mg/mL

technique(s)

indirect ELISA: 1:100,000, quantitative precipitin assay: 2.5-5 mg/mL

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

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General description

IgA is the highest generated antibody isotype. IgAs are mainly secreted in the intestinal mucosa.
Anti-Human IgA (α-chain specific) antibody is specific for the α-chain of IgA in humans. The product reacts with human IgA when tested against purified human IgA, IgG, IgM, and Bence Jones κ and lambda myeloma proteins.
Immunoglobulin A (IgA) exists in two isotypic forms: IgA1 and IgA2. It is produced as secretory IgA (sIgA) and serum IgA. sIgA exists in a monomeric form with two heavy and two light chains. Serum IgA exists in the dimeric form linked by disulfide bind with an additional J chain. IgA is found in saliva, serum, colostrum and nasal fluid.

Specificity

The antibody preparation is specific for human IgA when tested against purified human IgA, IgG, IgM, Bence Jones kappa and lambda myeloma proteins.

Immunogen

IgA purified from normal human colostrum

Application

Anti-Human IgA (α-chain specific) antibody is suitable for use in:
  • enzyme-linked immunosorbent assay (ELISA)
  • quantitative precipitin assay (2.5-5 mg/mL)
  • immunoelectrophoresis
  • Ouchterlony double diffusion (1:16)

Biochem/physiol Actions

IgA is a secretory glycoprotein antibody that regulates the physiological balance between commensal intestinal bacteria and the defenses of the host immune system. It prevents the commensal microorganisms from invading the intestines.
Immunoglobulin A (IgA) mediates antibody-dependent cell-mediated cytotoxicity (ADCC) by binding to leukocytes and enhances microbial destruction. It acts as the first line of defense in antigen clearance. IgA initiates the alternative pathway of the complement system to perform protective functions. It reduces inflammation stimulated by the classical pathway complement activation. IgA prevents proteolysis and inhibits the uptake of bacterial, viral or antigen attachment.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Salivary IgA from the sublingual compartment as
a novel noninvasive proxy for intestinal immune
induction
Aase A, et al.
Mucosal Immunology, 9(4), 884-884 (2016)
Salivary Immuno Factors, Cortisol and
Testosterone Responses in Athletes of a
Competitive 5,000 m Race
Li CY, et al.
The Chinese Journal of Physiology, 58(4), 263-269 (2015)
The structure and function of human IgA.
M A Kerr
The Biochemical journal, 271(2), 285-296 (1990-10-15)
A J Macpherson
Current topics in microbiology and immunology, 308, 117-136 (2006-08-23)
The lower intestine of mammals is colonised by a dense flora composed mainly of non-pathogenic commensal bacteria. These intestinal bacteria have a wide-ranging impact on host immunity and physiology. One adaptation following intestinal colonisation is increased production and secretion of
Emma Slack et al.
Frontiers in immunology, 3, 100-100 (2012-05-09)
Intestinal bacteria outnumber our own human cells in conditions of both health and disease. It has long been recognized that secretory antibody, particularly IgA, is produced in response to these microbes and hypothesized that this must play an important role

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