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Millipore

Rhamnose MacConkey (VTEC O26) Agar

suitable for microbiology, NutriSelect® Basic

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Synonym(s):
RMAC

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 12.0 g/L
bile salts No. 3, 1.5 g/L
crystal violet, 0.001 g/L
neutral red, 0.03 g/L
peptone, 15.0 g/L
rhamnose, 20.0 g/L
sodium chloride, 5.0 g/L

manufacturer/tradename

NutriSelect® Basic

final pH

7.1±0.2 (25 °C)

application(s)

clinical
environmental
food and beverages

microbiology

suitability

enterovirulent E. coli (EEC)

Related Categories

Application

This medium is selective for the isolation of the verocytotoxin producing Escherichia coli O26. This strain has been associated with haemorrhagic colitis (HC) and haemolytic uraemic syndrome (HUS). It is based on the MacConkey formulation No.3, where the fermentable carbohydrate of lactose has been substituted for rhamnose. VTEC O26 colonies are not able to ferment the rhamnose so will remain a translucent colour on the medium. All other non-O26 VTEC colonies present are able to ferment Rhamnose and will appear as pink to red colonies. Selectivity of the medium can be increased by adding Cefixime Tellurite (CT) supplement.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


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