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GranuCult® prime Sorbitol MacConkey (SMAC) Agar

acc. FDA-BAM, ISO 16654

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Synonym(s):
CT-SMAC Agar, MacConkey Agar with Sorbitol, SMAC Agar

agency

ISO 16654

Quality Level

form

powder

technique(s)

microbiological culture: suitable

pH

7.1±0.2 (25 °C)

application(s)

food and beverages
pharmaceutical

storage temp.

15-25°C

suitability

selective and differential for Escherichia 0157:H7

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This Item
1.002051.038531.46022
vibrant-m

1.46022

HEIMPLATE MacConkey agar

storage temp.

15-25°C

storage temp.

-

storage temp.

15-25°C

storage temp.

15-25°C

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

agency

ISO 16654

agency

according to EP, according to ISO (21150, 21567, FDA-BAM ), according to JP, according to USP

agency

ISO 6579

agency

EP 2.6.13, JP 4.05, USP 62

form

powder

form

powder

form

powder

form

plate (ready-to-use)

pH

7.1±0.2 (25 °C)

pH

-

pH

7.0±0.2 (25 °C)

pH

7.1 ( in H2O)

General description

GranuCult® prime Sorbitol MacConkey (SMAC) Agar is a selective agar medium to isolate and differentiate enterohemorrhagic (EHEC) E. coli 0157 from food, animal feed and other samples.
The presence of bile salts and crystal violet inhibits the growth of gram-positive microbes thereby enhancing the growth of gram-negative microorganisms.
The specific selectivity of the medium is done by the addition of Cefixime potassium Tellurite supplement towards E. coli 0157:H7 and in turn suppresses the accompanying flora. Addition of sorbitol in the medium along with pH indicator neutral red is used to detect the sorbitol positive colonies which appear red in color. Typical colonies of (sorbitol negative) E. coli 0157 are transparent, colorless to pale yellowish with small diameter.
Enzymatic digest of casein and animal tissue provides nitrogen, vitamins, and amino acids to the growing microbes. Agar is the solidifying agent.

Application

GranuCult® prime Sorbitol MacConkey (SMAC) Agar is used for the isolation and differentiation of sorbitol negative Escherichia coli serotype O157 from food, animal feed and other test samples.

Analysis Note

  • The dehydrated media is granulated with light pink color.
  • Typical colonies of (sorbitol negative) E. coli 0157 are transparent, colorless to pale yellowish with small diameter.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


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