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75977

Millipore

PALCAM Listeria Selective Agar

NutriSelect® Plus, suitable for microbiology

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NACRES:
NA.85

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 13.0 g/L
ammonium ferric citrate, 0.5 g/L
esculin, 0.8 g/L
glucose, 0.5 g/L
lithium chloride, 15.0 g/L
D-mannitol, 10.0 g/L
peptone, 23.0 g/L
phenol red, 0.08 g/L
sodium chloride, 5.0 g/L
starch, 1.0 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.0±0.2 (25 °C)

application(s)

agriculture
environmental
food and beverages
pathogen testing
veterinary

microbiology

suitability

selective and differential for Listeria spp.

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PALCAM Listeria Selective Agar NutriSelect® Plus, suitable for microbiology

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PALCAM Listeria Selective Agar

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suitability

selective and differential for Listeria spp.

suitability

selective and differential for Listeria spp.

suitability

selective and differential for Listeria spp.

suitability

nonselective for Bacillus spp., nonselective for Escherichia coli, nonselective for Lactobacillus spp., nonselective for Lactococcus spp., nonselective for Listeria spp., nonselective for Staphylococcus spp., nonselective for Streptococcus spp., nonselective for bacteria (General Media), nonselective for coliforms

form

powder

form

powder

form

powder

form

powder

composition

agar, 13.0 g/L , esculin, 0.8 g/L , lithium chloride, 15.0 g/L , peptone, 23.0 g/L , sodium chloride, 5.0 g/L , ammonium ferric citrate, 0.5 g/L , D-mannitol, 10.0 g/L , starch, 1.0 g/L , glucose, 0.5 g/L , phenol red, 0.08 g/L

composition

agar, 15.0 g/L , casein enzymic hydrolysate, 6.0 g/L , chromogenic substrate, 0.05 g/L , disodium hydrogen phosphate anhydrous, 2.5 g/L , glucose, 2.0 g/L , lithium chloride, 10.0 g/L , magnesium glycerophosphate, 1.0 g/L , magnesium sulfate, 0.5 g/L , meat peptone, 18.0 g/L , sodium chloride, 5.0 g/L , sodium pyruvate, 2.0 g/L , yeast extract, 10.0 g/L

composition

agar, 10 g/L , corn starch, 1 g/L , esculin, 1 g/L , ferric ammonium citrate, 0.5 g/L , lithium chloride, 15 g/L , sodium chloride, 5 g/L , special peptone, 23 g/L

composition

agar, 9.0 g/L , dextrose, 1.0 g/L , tryptone, 5.0 g/L , yeast extract, 2.5 g/L

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

application(s)

agriculture
environmental
food and beverages
pathogen testing
veterinary, microbiology

application(s)

agriculture
environmental
food and beverages, microbiology

application(s)

agriculture
environmental
food and beverages
veterinary, microbiology

application(s)

agriculture
bioburden testing
environmental
food and beverages
water monitoring, microbiology

General description

Polymyxin acriflavine lithium chloride ceftazidime aesculin mannitol (PALCAM) Listeria Selective Agar is a selective medium. Selectivity is attained mainly by the inhibitory components like lithium chloride, acriflavine, polymyxin and ceftazidime. Two indicator systems confer its diagnostic properties: aesculin and ferric iron and D-mannitol combined with phenol red. This aids in easy differentiation of Listeria spp. from enterococci and staphylococci.

Application

for differential diagnosis, for the isolation and detection of Listeria monocytogenes from fecal and biological material, food and heavily contaminated environmental specimens.

Preparation Note

Dissolve 68.8 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool down to 50°C and add the dissolved contents of 2 vials PALCAM Listeria Selective Supplement (Cat. No. 02336). The completed broth may appear hazy due to starch.

Other Notes

Detection of Listeria species in a range of meat products

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


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Customers Also Viewed

Slide 1 of 3

1 of 3

C.P.G.L. Gunasinghe et al.
Letters in Applied Microbiology, 18, 156-156 (1994)
Polymyxin acriflavine lithium chloride ceftazidime aesculin mannitol (PALCAM) agar
Progress in Industrial Microbiology, 395-398 (1995)
Van Nettern, P., et al.
Letters in Applied Microbiology, 12, 20-20 (1991)

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