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ChromoCult® Listeria Agar Enrichment-Supplement

ChromoCult®, selective for Listeria spp.

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Synonym(s):
Listeria Enrichment Supplement

agency

APHA
ISO 11133
ISO 11290-1
ISO 11290-2

Quality Level

form

lyophilized powder

manufacturer/tradename

ChromoCult®

technique(s)

microbiological culture: suitable

pH

6.6 (20 °C in H2O)

application(s)

food and beverages
microbiology
pathogen testing

storage temp.

2-8°C

suitability

selective for Listeria spp. (Agar)

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This Item
1.004271.117811.07006
suitability

selective for Listeria spp. (Agar)

suitability

Listeria spp.

suitability

Listeria spp.

suitability

selective for Listeria spp.

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

application(s)

food and beverages
microbiology
pathogen testing

application(s)

food and beverages
microbiology

application(s)

food and beverages
microbiology

application(s)

food and beverages
microbiology

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

2-8°C

storage temp.

15-25°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

ChromoCult® Listeria Agar Enrichment-Supplement is a homogeneous, sterile suspension of L-α-phosphatidylinositol in distilled water. Differentiation of Listeria monocytogenes from non-pathogenic Listeria sp. is achieved through the production of a phosphatidylinositol-specific phospholipase C (PI-PLC). Listeria monocytogenes hydrolyses the specific purified substrate added to the medium producing an opaque halo around the colonies.

Application

ChromoCult® Listeria Agar Enrichment-Supplement is used as an additive for the preparation of ChromoCult® Listeria Selective Agar for detection and enumeration of Listeria monocytogenes and of Listeria sp. in a variety of food products.

Linkage

Replaces: 1004390017

Analysis Note

Growth promotion test according to ISO 11133.
Inoculum on reference medium
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
10 - 100
- Listeria monocytogenes ATCC 19111 (WDCM 00020): 10 - 100
- Listeria innocua ATCC 33090 (WDCM 00017): 10 - 100
Recovery on test medium
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
≥ 70 %
- Listeria monocytogenes ATCC 19111 (WDCM 00020): ≥ 70 %
- Listeria innocua ATCC 33090 (WDCM 00017): ≥ 70 %
Colonies
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
blue-green; non-transparent zone
- Listeria monocytogenes ATCC 19111 (WDCM 00020): blue-green; non-transparent zone
- Listeria innocua ATCC 33090 (WDCM 00017): blue-green
Growth
- Escherichia coli ATCC 25922 (WDCM 00013):
no growth
- Escherichia coli ATCC 8739 (WDCM 00012): no growth
- Enterococcus faecalis ATCC 19433 (WDCM 00009): no growth
- Enterococcus faecalis ATCC 29212 (WDCM 00087): no growth
Incubation: Listeria 24-48 Std. 37°C aerobic; other strains at least 48 hrs.

Legal Information

CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Articles

Detection of Listeria monocytogenes in food and environmental samples according to ISO 11290-1, FDA-BAM and USDA-FSIS

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