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ChromoCult® Listeria agar (base) acc. OTTAVIANI and AGOSTI

ISO 11290, For the isolation and differentiation of Listeria monocytogenes and other Listeria sp.

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Synonym(s):
Listeria selective agar

agency

APHA
ISO 11290

Quality Level

reg. compliance

FDA (BAM)

form

medium granules (dehydrated (DCM))

manufacturer/tradename

ChromoCult®

technique(s)

microbiological culture: suitable

pH

7.0-7.4 (25 °C, 70 g/L in H2O)

solubility

70 g/L

application(s)

food and beverages
microbiology

storage temp.

15-25°C

suitability

Listeria spp.

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GranuCult® Oxford Agar (Base)

form

medium granules (dehydrated (DCM))

form

lyophilized powder

form

powder (dehydrated (DCM))

form

medium granules (dehydrated (DCM))

suitability

Listeria spp.

suitability

selective for Listeria spp. (Agar)

suitability

Listeria spp.

suitability

Listeria spp. (spp.)

agency

APHA, ISO 11290

agency

APHA, ISO 11133, ISO 11290-1, ISO 11290-2

agency

ISO 11290

agency

ISO 11290

application(s)

food and beverages
microbiology

application(s)

food and beverages
microbiology
pathogen testing

application(s)

food and beverages
microbiology

application(s)

food and beverages
microbiology

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

200

General description

Listeria agar acc. OTTAVIANI and AGOSTI contains the chromogenic compound 5-bromo-4-chloro-3-indolyl-β-D-glucopyranoside, which is a substrate for the detection of β-glucosidase. This enzyme is common in all Listeria that appears as blue-green colored colonies on the medium. Differentiation of Listeria monocytogenes from non-pathogenic Listeria sp. is achieved through the production of a phosphatidylinositol-specific phospholipase C (PI-PLC). Listeria monocytogenes hydrolyses the specific purified substrate added to the medium producing an opaque halo around the colonies. Most Listeria ivanovii also produce an opaque halo around the colonies after 48 h incubation. The inhibitors in the medium reduce the growth of the majority of concomitant gram-positive and gram-negative pathogens, as well as of yeasts and fungi. Agar is the solidifying agent, while lithium chloride, nalidixic acid, and cycloheximide provide selectivity.

Application

ChromoCult® Listeria agar (base) is used for the isolation and differentiation of Listeria monocytogenes and other Listeria sp. from food and animal feed, environmental samples in the area of food production and food handling, and other materials.

Analysis Note

Appearance (colour): yellowish
Appearance (clearness): slightly opalescent to opalescent
pH-value (25 °C): 7.0 - 7.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)):
Colony count (Listeria monocytogenes ATCC 13932 (WDCM 00021)):
Recovery on test medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)): ≥ 50 %
Colonies (Listeria monocytogenes ATCC 13932 (WDCM 00021)): blue green colonies with opaque halo
Inoculum on reference medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)):
Colony count (Listeria monocytogenes ATCC 35152 (WDCM 00109)):
Recovery on test medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)): ≥ 50 %
Colonies(Listeria monocytogenes ATCC 35152 (WDCM 00109)): blue green colonies with opaque halo
Growth (Listeria innocua ATCC 33090 (WDCM 00017)): no limit
Colonies (Listeria innocua ATCC 33090 (WDCM 00017)): blue green colonies without opaque halo
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): total inhibition
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): total inhibition
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): total inhibition
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087)): total inhibition
Incubation: 44 ± 4 hours at 37 ± 1 °C
Reference media: Tryptic Soy Agar
A recovery rate of 50 % is equivalent to a productivity value of 0.5.
The indicated colony counts result from the sum of a triple determination.

Legal Information

CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

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Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


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