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1.11755

Millipore

GranuCult® PALCAM agar (Base)

pkg of 500 g, according to FDA (BAM), ISO 11290, for Listeria spp.

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Synonym(s):
Listeria selective agar

agency

ISO 11290

Quality Level

reg. compliance

according to FDA (BAM)

form

powder (dehydrated (DCM))

packaging

pkg of 500 g

manufacturer/tradename

GranuCult®

technique(s)

microbiological culture: suitable

pH

6.9-7.1 (25 °C, 68.8 g/L in H2O, after autoclaving)

solubility

68.8 g/L

bulk density

650 kg/m3

application(s)

food and beverages
microbiology

storage temp.

15-25°C

suitability

Listeria spp.

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This Item
1.00427759771.07004
vibrant-m

1.11755

GranuCult® PALCAM agar (Base)

vibrant-m

75977

PALCAM Listeria Selective Agar

vibrant-m

1.07004

GranuCult® Oxford Agar (Base)

suitability

Listeria spp.

suitability

Listeria spp.

suitability

selective and differential for Listeria spp.

suitability

Listeria spp. (spp.)

form

powder (dehydrated (DCM))

form

medium granules (dehydrated (DCM))

form

powder

form

medium granules (dehydrated (DCM))

agency

ISO 11290

agency

APHA, ISO 11290

agency

-

agency

ISO 11290

application(s)

food and beverages
microbiology

application(s)

food and beverages
microbiology

application(s)

agriculture
environmental
food and beverages
pathogen testing
veterinary, microbiology

application(s)

food and beverages
microbiology

solubility

68.8 g/L

solubility

70 g/L

solubility

-

solubility

58.5 g/L

General description

GranuCult® PALCAM agar (Base) provides quantitative cultivation of Listeria monocytogenes and inhibits the accompanying Gram-negative and most of the Gram-positive bacteria. Polymyxin, acriflavin, ceftazidime, and lithium chloride result in the selectivity of the medium. L. monocytogenes break down the esculin in the medium to glucose and esculetin. Esculetin forms an olive-green to black complex with iron (III) ions, which stains the colonies of L. monocytogenes. Mannitol-positive accompanying bacteria such as Staphylococci grow as yellow colonies if they are not inhibited.

Application

GranuCult® PALCAM agar (Base) is suitable for the detection and isolation of Listeria monocytogenes from feces, biological samples, foodstuffs, and heavily contaminated material from the environment.

Analysis Note

Base tested with PALCAM-Listeria selective supplement acc. to VAN NETTEN et.al. art. no. 1.12122.
Appearance (clearness): clear to slightly opalescent
Appearance (color): red
pH-value (25 °C): 7.0 - 7.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)):
Inoculum on reference medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)):
Inoculum on reference medium (Listeria innocua ATCC 33090 (WDCM 00017)):
Colony count (Listeria monocytogenes ATCC 35152 (WDCM 00109)):
Colony count (Listeria monocytogenes ATCC 13932 (WDCM 00021)):
Colony count (Listeria innocua ATCC 33090 (WDCM 00017)):
Recovery on test medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)): ≥ 50 %
Recovery on test medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)): ≥ 50 %
Recovery on test medium (Listeria innocua ATCC 33090 (WDCM 00017)): ≥ 50 %
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): total inhibition
Growth(Escherichia coli ATCC 8739 (WDCM 00012)): total inhibition
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): partial inhibition
Black zone (Listeria monocytogenes ATCC 35152 (WDCM 00109)): black zone
Black zone (Listeria monocytogenes ATCC 13932 (WDCM 00021)): black zone
Black zone (Listeria innocua ATCC 33090 (WDCM 00017)): black zone
Incubation: up to 48 hours at 35 ± 1 °C, aerobic
A recovery rate of 50 % is equivalent to a productivity value of 0.5.
The indicated colony counts result from the sum of a triple determination.
Reference media: Tryptic Soy Agar

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark

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Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


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Articles

The different types of culture media, that are used to grow microorganisms in the laboratory for quality control, are classified by several criteria, such as consistency, composition, or selectivity.

Detection of Listeria monocytogenes in food and environmental samples according to ISO 11290-1, FDA-BAM and USDA-FSIS

Protocols

In recent years it has been recognized that Listeria is an important public health problem. The disease affects primarily people of advanced age, pregnant women, newborns, and adults with weakened immune systems.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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