MilliporeSigma
All Photos(1)

Documents

1.07004

Millipore

GranuCult® Oxford Agar (Base)

pkg of 500 g, according to FDA (BAM), ISO 11290, for Listeria spp.

Sign Into View Organizational & Contract Pricing

Synonym(s):
Listeria selective agar

agency

ISO 11290

Quality Level

reg. compliance

according to FDA (BAM)

form

medium granules (dehydrated (DCM))

packaging

pkg of 500 g

manufacturer/tradename

GranuCult®

technique(s)

microbiological culture: suitable

pH

6.9-7.1 (35 °C, 58.5 g/L in H2O, after autoclaving)

solubility

58.5 g/L

bulk density

810 kg/m3

application(s)

food and beverages
microbiology

storage temp.

15-25°C

suitability

Listeria spp. (spp.)

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
1.00427758051.11755
GranuCult® Oxford Agar (Base) pkg of 500 g, according to FDA (BAM), ISO 11290, for Listeria spp.

1.07004

GranuCult® Oxford Agar (Base)

Oxford Agar suitable for microbiology, NutriSelect® Plus

75805

Oxford Agar

GranuCult® PALCAM agar (Base) pkg of 500 g, according to FDA (BAM), ISO 11290, for Listeria spp.

1.11755

GranuCult® PALCAM agar (Base)

form

medium granules (dehydrated (DCM))

form

medium granules (dehydrated (DCM))

form

powder

form

powder (dehydrated (DCM))

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

suitability

Listeria spp. (spp.)

suitability

Listeria spp.

suitability

selective and differential for Listeria spp.

suitability

Listeria spp.

application(s)

food and beverages
microbiology

application(s)

food and beverages
microbiology

application(s)

agriculture
environmental
food and beverages
veterinary, microbiology

application(s)

food and beverages
microbiology

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

-

storage temp.

15-25°C

General description

GranuCult® Oxford Agar (Base) formulation is based on Columbia Agar with the addition of lithium chloride, acriflavin, colistin sulfate, cefotetan, cycloheximide, and fosfomycin. These ingredients suppress the growth of the common bacteria (e.g., Gram-negative bacteria and a greater part of Gram-positive bacteria). Lithium chloride is one of the ingredients of Oxford Agar base, while the other substances are derived from the Oxford Listeria Selective Supplement (1.07006). Listeria monocytogenes hydrolyses esculin to esculetin and forms a black complex with iron (III) ions, thereby producing brown-green colored colonies with a black halo.

Application

GranuCult® Oxford Agar (Base) is a selective agar for the isolation and detection of Listeria monocytogenes in milk and milk products and other food products.

Analysis Note

Base tested with Oxford-Listeria-selektiv-supplement art. no. 1.07006.
Appearance (clearness): clear
Appearance (color): blueish-brown to greenish-brown
pH-value (25 °C): 6.8 - 7.2
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)):
Inoculum on reference medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)):
Inoculum on reference medium (Listeria innocua ATCC 33090 (WDCM 00017)):
Colony count (Listeria monocytogenes ATCC 35152 (WDCM 00109)):
Colony count (Listeria monocytogenes ATCC 13932 (WDCM 00021)):
Colony count (Listeria innocua ATCC 33090 (WDCM 00017)):
Recovery on test medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)): ≥ 50
Recovery on test medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)): ≥ 50
Recovery on test medium (Listeria innocua ATCC 33090 (WDCM 00017)): ≥ 50
Growth Escherichia coliATCC 25922 (WDCM 00013): total inhibition
Growth(Escherichia coli ATCC 8739 (WDCM 00012)): total inhibition
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): partial inhibition
Growth (Candida albicans ATCC 10231 (WDCM 00054)): partial inhibition
Black zone (Listeria monocytogenes ATCC 35152 (WDCM 00109)): black zone
Black zone (Listeria monocytogenes ATCC 13932 (WDCM 00021)): black zone
Black zone (Listeria innocua ATCC 33090 (WDCM 00017)): black zone
Incubation: up to 48 hours at 35 ± 1 °C, aerobic
A recovery rate of 50 % is equivalent to a productivity value of 0.5.
The indicated colony counts result from the sum of a triple determination.
Reference media: Tryptic Soy Agar

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Articles

Microbial culture media is available in both powdered and granulated forms. This article compares the characteristics of each culture media format with regards to safety, handling and convenience.

Detection of Listeria monocytogenes in food and environmental samples according to ISO 11290-1, FDA-BAM and USDA-FSIS

Protocols

Listeria is a public health concern, affecting elderly, pregnant, newborns, and immunocompromised individuals.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service