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70139

Millipore

Potato Dextrose Agar

NutriSelect® Plus, suitable for microbiology

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Synonym(s):
PDA, Potato Glucose Agar, Potato Glucose Agar
NACRES:
NA.74

agency

APHA
USP

Quality Level

reg. compliance

FDA

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L
dextrose, 20 g/L
potato extract, 4 g/L

packaging

pkg of 100 g
pkg of 2.5 kg
pkg of 500 g

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

color

light yellow

final pH

5.6±0.2 (25 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages

microbiology

suitability

nonselective for Aspergillus spp.
nonselective for Candida spp.
nonselective for Penicillium spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.
nonselective for molds (General Media)
nonselective for yeasts (General Media)

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This Item
172048858870185
vibrant-m

70139

Potato Dextrose Agar

vibrant-m

88588

Plate-Count-Agar (Agar 15 g/L)

vibrant-m

70185

Czapek Dox Agar

suitability

nonselective for Aspergillus spp., nonselective for Penicillium spp., nonselective for Saccharomyces spp., nonselective for molds (General Media), nonselective for Candida spp., nonselective for Zygosaccharomyces spp., nonselective for Pichia spp., nonselective for yeasts (General Media)

suitability

selective for Aspergillus spp., selective for Candida spp., selective for Pichia spp., selective for Saccharomyces spp., selective for Zygosaccharomyces spp., selective for molds (General Media), selective for yeasts (General Media)

suitability

nonselective for Bacillus spp., nonselective for Enterococcus spp., nonselective for Escherichia coli, nonselective for Lactococcus spp., nonselective for Staphylococcus spp., nonselective for Streptococcus spp., nonselective for bacteria (General Media), nonselective for coliforms

suitability

Arthrobacter spp., nonselective for Aspergillus spp., nonselective for Bacillus spp., nonselective for Candida spp., nonselective for Penicillium spp., nonselective for Saccharomyces spp., nonselective for Zygosaccharomyces spp., nonselective for molds (General Media), nonselective for yeasts (General Media)

agency

APHA, USP

agency

-

agency

according to GB 4789.2-2016, according to ISO 17410:2019, according to ISO 4833-1:2013, according to ISO 4833-2:2013

agency

-

form

powder

form

powder

form

powder

form

powder

composition

agar, 15 g/L , potato extract, 4 g/L , dextrose, 20 g/L

composition

agar, 15 g/L , dextrose (glucose), 20 g/L , potatoes infusion, 200 g/L , rose bengale, 0.0084 g/L

composition

agar, 15 g/L , casein enzymic hydrolysate, 5 g/L , dextrose, 1 g/L , yeast extract, 2.5 g/L

composition

agar, 15.0 g/L , di-potassium hydrogen phosphate, 1.0 g/L , iron(II) sulfate heptahydrate, 0.01 g/L , magnesium sulfate heptahydrate, 0.5 g/L , potassium chloride, 0.5 g/L , sodium nitrate, 3.0 g/L , sucrose, 30.0 g/L

sterility

non-sterile

sterility

-

sterility

non-sterile

sterility

non-sterile

General description

Potato Dextrose Agar is recommended for the isolation and enumeration of yeasts and molds in dairy and food products. Potato extract serves as a source of carbon, nitrogen, minerals, vitamins, and other essential growth nutrients. Dextrose acts as a source of carbohydrates. Agar is the solidifying agent. The accompanying bacterial flora is suppressed by the low pH of 3.5. The growth of yeasts and molds is promoted in this medium, and the fungi develop typical morphology. The addition of Rose Bengal further suppresses the growth of bacteria and restricts the size and spreading of mold colonies.

Application

Potato Dextrose Agar is used for the isolation and enumeration of yeasts and molds in dairy and food products. The media is also used for stimulating sporulation, maintaining stock cultures of certain dermatophytes, and as a differential media for certain varieties of dermatophytes on the basis of pigment production.

Features and Benefits

NutriSelect® Plus are powdered culture media offering
  • Quality control with growth promotion test according to industry standards
  • Quality assurance acc. to ISO 9001
  • Cost-efficient powdered media

Preparation Note

Suspend 39 g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. In order to suppress bacterial growth, 1 ml of 10% sterile lactic acid can be added for dropping pH to 3.5. Mix well before pouring.

Other Notes

Studies on contamination of yogurt by yeast; For maintaining the stock culture of Rhodotorula glutinis for the production of extracellular lipase; Identification of Aspergillus niger from infected onion tissue

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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M. Dublin Green et al.
Journal of Food Protection, 50, 193-193 (1987)
R.E. Beever et al.
Journal of General Microbiology, 60, 273-273 (1970)
A. Behere et al.
Enzyme and Microbial Technology, 15, 158-158 (1993)
D. Papaparaskevas et al.
Biotechnology Letters, 14, 397-397 (1992)
Julia Schumacher et al.
Eukaryotic cell, 7(4), 584-601 (2008-02-12)
Recently, we showed that the alpha subunit BCG1 of a heterotrimeric G protein is an upstream activator of the Ca(2+)/calmodulin-dependent phosphatase calcineurin in the gray mold fungus Botrytis cinerea. To identify the transcription factor acting downstream of BCG1 and calcineurin

Articles

Yeast is one of the most important microorganisms known and utilised by mankind. Ancient Middle Eastern civilisations used the organism to bake bread and to produce mead, beer and wine.

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