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68059

Sigma-Aldrich

FITC-CM-Dextran

average mol wt 4,000

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Synonym(s):
FITC-CM-Dextran 4, Fluorescein isothiocyanate–Carboxymethyl–Dextran, Fluorescein isothiocyanate–Carboxymethyl–Dextran
NACRES:
NA.32

biological source

synthetic

Quality Level

conjugate

FITC conjugate

form

powder or crystals

mol wt

average mol wt 4,000

composition

carboxymethyl content, 3-7%

extent of labeling

0.001-0.020 mol/mol FITC glucose

technique(s)

permeability assay: suitable

fluorescence

λex 493 nm; λem 517 nm±5 nm in 0.1 M Tris pH 8.0

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1 of 4

This Item
SAB42007387481754702
vibrant-m

68059

FITC-CM-Dextran

vibrant-m

74817

FITC-CM-Dextran

vibrant-m

54702

FITC–DEAE–Dextran

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100

technique(s)

permeability assay: suitable

technique(s)

immunoblotting: 1.25-2.5 μg/mL using ExtrAvidin-FITC, immunohistochemistry: suitable, indirect ELISA: 0.15-0.3 μg/mL using 5 μg/ml ExtrAvidin-FITC

technique(s)

-

technique(s)

permeability assay: suitable

form

powder or crystals

form

buffered aqueous solution

form

powder or crystals

form

powder or crystals

conjugate

FITC conjugate

conjugate

-

conjugate

FITC conjugate

conjugate

FITC conjugate

mol wt

average mol wt 4,000

mol wt

-

mol wt

average mol wt 150,000

mol wt

average mol wt 70,000

General description

FITC-CM-Dextran, also known as Fluorescein isothiocyanate–Carboxymethyl–Dextran, is an anionic fluorescent probe. The carboxyl group provides the negative charge, which improves the overall permeability of the molecule. It is supplied as a yellow powder that is freely soluble in water and electrolyte solutions. FITC-CM-Dextran is insoluble in most organic samples such as ethanol, methanol, acetone, and chloroform. FITC-CM-Dextran is manufactured by reacting selected dextran fractions with an activated carboxymethyl derivative in alkali, whereby O-carboxymethyl groups are introduced along the dextran chain.

Application

FITC-CM-dextran is used to study drug intake and delivery mechanisms in HeLa cell lines. FITC-CM-Dextran was chosen due to its high water solubility and negative charge. It is also a suitable molecule to be used in encapsulation studies for polymersomal drug delivery systems or Ps DDS. FITC-CM-Dextran is a biocompatible molecule used as a starting material in several pharmaceutical and diagnostic applications.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Articles

Additional information about FITC-labelled polysaccarides including FITC-Dextran, FITC-DEAE-dextran (FDD), FITC-carboxymethyl-dextran (FCM-Dextran). This page discusses the impact of weight-average molecular weight on the respective molecule dimensions and other physical properties

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