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FD10S

Sigma-Aldrich

Fluorescein isothiocyanate–dextran

average mol wt 10,000

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Synonym(s):
FITC–Dextran
CAS Number:
MDL number:
NACRES:
NA.25

biological source

sugar beets root

Quality Level

conjugate

FITC conjugate

form

powder

mol wt

average mol wt 10,000

extent of labeling

0.003-0.020 mol FITC per mol glucose

color

yellow to yellow-orange

solubility

water: 25 mg/mL, cloudy to hazy, yellow to orange

storage temp.

2-8°C

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1 of 4

This Item
FD20SFD70SFD250S
form

powder

form

powder

form

powder

form

powder

color

yellow to yellow-orange

color

yellow to dark yellow

color

yellow to yellow-orange

color

yellow to orange

solubility

water: 25 mg/mL, cloudy to hazy, yellow to orange

solubility

water: 25 mg/mL

solubility

H2O: 25 mg/mL, clear to slightly hazy, yellow-orange

solubility

water: 25 mg/mL, clear, yellow

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

Application

Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.

Preparation Note

Sigma typically tests the solubility of FITC dextrans in water at concentrations at or above 25 mg/ml. Solutions should be protected from light. In vivo, FITC-dextran is stable for more than 24 hours.

Other Notes

Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.
To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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The structure of NRRL B-512F dextran, methylation studies.
Van Cleve JW, Schacfer WC and Rist CE
Journal of the American Chemical Society, 78(17), 4435-4438 (1956)
Antonini, E. et al.
Biopolymers null
Gabriela Hurtado-Alvarado et al.
PloS one, 11(11), e0167236-e0167236 (2016-11-29)
Chronic sleep restriction induces blood-brain barrier disruption and increases pro-inflammatory mediators in rodents. Those inflammatory mediators may modulate the blood-brain barrier and constitute a link between sleep loss and blood-brain barrier physiology. We propose that adenosine action on its A2A
Lu Yuan et al.
Frontiers in microbiology, 11, 599555-599555 (2020-12-18)
Mechanisms of gastrointestinal protection by probiotic bacteria against infection involve amongst others, modulation of intestinal epithelial barrier function. Trans-epithelial electrical resistance (TEER) is widely used to evaluate cellular barrier functions. Here, we developed a two-stage interpretative model of the time-dependence
O Baron-Epel et al.
Planta, 175(3), 389-395 (1988-09-01)
The non-invasive technique of fluorescence redistribution after photobleaching was employed on soybean (Glycine max (L.) Merr.) root cells grown in suspension culture to examine macromolecular transport across plant cell walls. Using both fluorescently derivatized dextrans and proteins of graded size

Related Content

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran.

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