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FD150S

Sigma-Aldrich

Fluorescein isothiocyanate–dextran

average mol wt 150,000

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Synonym(s):
FITC–Dextran
CAS Number:
MDL number:
NACRES:
NA.25

biological source

synthetic

Quality Level

conjugate

FITC conjugate

form

powder

mol wt

average mol wt 150,000

extent of labeling

0.003-0.025 mol FITC per mol glucose

color

faint yellow to orange

solubility

H2O: 25 mg/mL, clear to slightly hazy, yellow-orange

storage temp.

2-8°C

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This Item
FD2000SFD250SFD20S
form

powder

form

powder

form

powder

form

powder

color

faint yellow to orange

color

yellow to orange

color

yellow to orange

color

yellow to dark yellow

solubility

H2O: 25 mg/mL, clear to slightly hazy, yellow-orange

solubility

H2O: soluble 25 mg/mL

solubility

water: 25 mg/mL, clear, yellow

solubility

water: 25 mg/mL

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

General description

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran. Conflicting data on the branch lengths implies that the average branch length is less than three glucose units. However, other methods indicate branches of greater than 50 glucose units exist. Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.

Application

Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.
FITC-dextran is used extensively in microcirculation and cell permeability research utilizing microfluorimetry. FITC-dextran has been used to study plant cell wall porosity and capillary permeability. Plasma proteins have been shown not to bind to FITC-dextran.
FITC-dextran is used in cardiovascular, microcirculation, perfusion, cell monolayer and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination. Small FITC-dextrans are also used to measure processes such as endocytosis and cell junction permeability. Fluorescein-dextran, 150 kDa may be used to help assess the integrity of the blood brain barrier (BBB) and to perform fluorescence microlymphography (FML). Fluorescein-dextran 150 is used as a microcirculation vascular flux tracer.

Preparation Note

Sigma typically tests the solubility of FITC dextrans in water at concentrations at or above 25 mg/ml. Solutions should be protected from light. In vivo, FITC-dextran is stable for more than 24 hours.

Other Notes

To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Jesús E Rojo Arias et al.
Journal of neurochemistry, 153(3), 390-412 (2019-09-25)
Retinal hypoxia triggers abnormal vessel growth and microvascular hyper-permeability in ischemic retinopathies. Whereas vascular endothelial growth factor A (VEGF-A) inhibitors significantly hinder disease progression, their benefits to retinal neurons remain poorly understood. Similar to humans, oxygen-induced retinopathy (OIR) mice exhibit
Luana Schito et al.
EMBO molecular medicine, 12(9), e11416-e11416 (2020-07-21)
Conventional maximum-tolerated dose (MTD) chemotherapy relies on periodic, massive cancer cell ablation events followed by treatment-free intermissions, stereotypically resulting in resistance, relapse, and mortality. Furthermore, MTD chemotherapy can promote metastatic dissemination via activation of a transcriptional program dependent on hypoxia-inducible
Guillaume Tetreau et al.
Nature communications, 11(1), 1153-1153 (2020-03-04)
Cyt1Aa is the one of four crystalline protoxins produced by mosquitocidal bacterium Bacillus thuringiensis israelensis (Bti) that has been shown to delay the evolution of insect resistance in the field. Limiting our understanding of Bti efficacy and the path to
Jiyun Kim et al.
Advanced materials (Deerfield Beach, Fla.), 28(1), 132-137 (2015-11-10)
Biomimetic extracellular matrix (ECM) topographies driven by the magnetic-field-directed self-assembly of ECM protein-coated magnetic beads are fabricated. This novel bottom-up method allows us to program isotropic, anisotropic, and diverse hybrid ECM patterns without changing other physicochemical properties of the scaffold
Haruo Aramoto et al.
American journal of physiology. Heart and circulatory physiology, 287(4), H1590-H1598 (2004-05-25)
Vascular endothelial growth factor (VEGF) induces mild vasodilation and strong increases in microvascular permeability. Using intravital microscopy and digital integrated optical intensity image analysis, we tested, in the hamster cheek pouch microcirculation, the hypothesis that differential signaling pathways in arterioles

Related Content

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran.

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