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FD20

Sigma-Aldrich

Fluorescein isothiocyanate–dextran

average mol wt 20,000

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Synonym(s):
FITC–Dextran
CAS Number:
MDL number:
NACRES:
NA.25

biological source

bacterial (Leuconostoc mesenteroides)

Quality Level

conjugate

FITC conjugate

form

powder

mol wt

average mol wt 20,000

extent of labeling

0.001-0.020 mol FITC per mol glucose

color

light yellow to dark orange

solubility

water: 25 mg/mL

storage temp.

2-8°C

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FD250SFD2000SFD20S
vibrant-m

FD20

Fluorescein isothiocyanate–dextran

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

solubility

water: 25 mg/mL

solubility

water: 25 mg/mL, clear, yellow

solubility

H2O: soluble 25 mg/mL

solubility

water: 25 mg/mL

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

color

light yellow to dark orange

color

yellow to orange

color

yellow to orange

color

yellow to dark yellow

form

powder

form

powder

form

powder

form

powder

General description

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran. Conflicting data on the branch lengths implies that the average branch length is less than three glucose units. However, other methods indicate branches of greater than 50 glucose units exist. Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.

Application

Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.
FITC-dextran is used extensively in microcirculation and cell permeability research utilizing microfluorimetry. FITC-dextran has been used to study plant cell wall porosity and capillary permeability. Plasma proteins have been shown not to bind to FITC-dextran.
FITC-dextran is used in cardiovascular, microcirculation, perfusion, cell monolayer and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination. Small FITC-dextrans are also used to measure processes such as endocytosis and cell junction permeability. Fluorescein-dextran, 20kDa, may be used to study cell processes such as endocytosis and osmoporation. FITC-dextran-20 may be used to study endothelial or epithelial monolayer porosity, apical-basolateral movements within cell layers and cell junction permeability. FITC-dextran-20 may be used as a model compound to study drug release from structures such as hydrogens and collagen minirods.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Preparation Note

Sigma typically tests the solubility of FITC dextrans in water at concentrations at or above 25 mg/ml. Solutions should be protected from light. In vivo, FITC-dextran is stable for more than 24 hours.

Other Notes

To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.

pictograms

Exclamation mark

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Markéta Gajdošová et al.
International journal of pharmaceutics, 592, 120086-120086 (2020-11-15)
The incidence of fungal infections has increased in recent decades not only in patients with predisposing and risk factors, but it has also spread up due to the widespread use of broad-spectrum antibiotics, immunosuppressants and corticosteroids. A limited number of
Barbara Zorec et al.
International journal of pharmaceutics, 516(1-2), 204-213 (2016-11-08)
We studied fractional Er:YAG laser to enhance transdermal drug delivery of compounds possessing different molecular weights: FITC-dextrans (or FD) with average molecular weights of 4, 10 and 20kDa. Vertical glass Franz diffusion cells were used to study molecular transport through
Dong-Xu Liu et al.
Neuroscience letters, 653, 189-194 (2017-06-04)
Sleep deprivation (SD) is a common condition associated with a variety of nervous system diseases, and has a negative impact on emotional and cognitive function. Continuous theta burst stimulation (cTBS) is known to improve cognition and emotion function in normal
Iris Metzmacher et al.
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 67(2), 349-360 (2007-04-06)
A drug delivery system, named minirod, containing insoluble non-cross-linked collagen was prepared to investigate the release of model drug compounds. To characterise the complete drug release process properly, a mathematical model was developed. Previously, a mathematical model describing water penetration
Damia Mawad et al.
International journal of pharmaceutics, 366(1-2), 31-37 (2008-09-24)
Injectable hydrogels have potential biomedical applications ranging from tissue fillers to drug delivery vehicles. This study focussed on evaluating the structure of poly(vinyl alcohol) (PVA) hydrogels of variable solid content and high molecular weight model drug release from the networks

Articles

Additional information about FITC-labelled polysaccarides including FITC-Dextran, FITC-DEAE-dextran (FDD), FITC-carboxymethyl-dextran (FCM-Dextran). This page discusses the impact of weight-average molecular weight on the respective molecule dimensions and other physical properties

Related Content

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran.

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