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FD250S

Sigma-Aldrich

Fluorescein isothiocyanate–dextran

average mol wt 250,000

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Synonym(s):
FITC–Dextran
CAS Number:
MDL number:

biological source

bacterial (Leuconostoc mesenteroides)

Quality Level

conjugate

FITC conjugate

form

powder

mol wt

average mol wt 250,000

extent of labeling

0.003-0.020 mol FITC per mol glucose

color

yellow to orange

solubility

water: 25 mg/mL, clear, yellow

storage temp.

2-8°C

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This Item
FD2000SFD500SFD40S
solubility

water: 25 mg/mL, clear, yellow

solubility

H2O: soluble 25 mg/mL

solubility

H2O: soluble 25 mg/mL, clear to slightly hazy, yellow to deep orange

solubility

H2O: soluble 25 mg/mL, clear to slightly hazy, yellow to deep orange

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

powder

form

powder

form

powder

form

powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

color

yellow to orange

color

yellow to orange

color

yellow to orange

color

yellow to dark orange

General description

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran. Conflicting data on the branch lengths implies that the average branch length is less than three glucose units. However, other methods indicate branches of greater than 50 glucose units exist. Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.

Application

Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.
FITC-dextran is used extensively in microcirculation and cell permeability research utilizing microfluorimetry. FITC-dextran has been used to study plant cell wall porosity and capillary permeability. Plasma proteins have been shown not to bind to FITC-dextran.
FITC-dextran is used in cardiovascular, microcirculation, perfusion, cell monolayer and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination. Small FITC-dextrans are also used to measure processes such as endocytosis. FITC-Dextran 250 (FD 250) may be used as a model compound for development of methods to deliver molecules into protoplasts and to study drug release from structures such as hydrogels and liposomes. It may be used as a model compound to study microvasculature and blood brain barrier.

Preparation Note

Sigma typically tests the solubility of FITC dextrans in water at concentrations at or above 25 mg/ml. Solutions should be protected from light. In vivo, FITC-dextran is stable for more than 24 hours.

Other Notes

To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.

pictograms

Exclamation mark

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Jennifer L Guerriero et al.
Nature, 543(7645), 428-432 (2017-03-09)
Although the main focus of immuno-oncology has been manipulating the adaptive immune system, harnessing both the innate and adaptive arms of the immune system might produce superior tumour reduction and elimination. Tumour-associated macrophages often have net pro-tumour effects, but their
Cristiana Marcozzi et al.
Regenerative medicine, 15(9), 2085-2098 (2020-11-18)
Aim: The proposal of this study was to evaluate, in vitro, the potential paracrine effect of human adipose-derived stem cells (hASCs) to promote lymphangiogenesis in lymphatic endothelial cells isolated from rat diaphragmatic lymphatic vessels. Materials & methods: ELISA on VEGFA, VEGFC and
Fabiana Gomes da Conceição et al.
Clinics (Sao Paulo, Brazil), 67(8), 923-929 (2012-09-06)
Ischemic preconditioning and some drugs can protect tissues from injury by preserving microcirculation. This study evaluated vascular permeability in a hamster cheek pouch preparation using either short ischemic periods or bradykinin as preconditioning stimuli followed by 30 min of ischemia/reperfusion.
Cerec Omnicam - Intraoralscan 2.0.
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International journal of computerized dentistry, 15(3), 199-205 (2012-12-21)
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Early stage growth of intracranial B16F10 tumors is reduced by 87% in myeloid-specific NG2 null (Mac-NG2ko) mice and by 77% in pericyte-specific NG2 null (PC-NG2ko) mice, demonstrating the importance of the NG2 proteoglycan in each of these stromal compartments. In

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Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran.

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