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Sigma-Aldrich

Fluorescein isothiocyanate – Dextran 500000-Conjugate

(FITC:Glucose = 1:100)

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Synonym(s):
Dextran 500′000 Fluorescein isothiocyanate Conjugate
NACRES:
NA.32

form

powder or crystals

Quality Level

composition

(FITC:Glucose = 1:100)

extent of labeling

0.01 mol/mol FITC glucose (substitution)

technique(s)

permeability assay: suitable

solubility

H2O: 25 mg/mL, clear to very faintly turbid, yellow to orange

fluorescence

λex 490 nm; λem 520 nm (pH 8.0)

storage temp.

2-8°C

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vibrant-m

53379

FITC-CM-Dextran

vibrant-m

74817

FITC-CM-Dextran

fluorescence

λex 490 nm; λem 520 nm (pH 8.0)

fluorescence

λex 493 nm; λem 517 nm±5 nm in 0.1 M Tris pH 8.0

fluorescence

λex 493 nm; λem 517 nm±5 nm in 0.1 M Tris pH 8.0

fluorescence

λex 498 nm; λem 521 nm in 0.1 M phosphate pH 7.0

solubility

H2O: 25 mg/mL, clear to very faintly turbid, yellow to orange

solubility

-

solubility

-

solubility

-

form

powder or crystals

form

powder or crystals

form

powder or crystals

form

powder

storage temp.

2-8°C

storage temp.

-

storage temp.

-

storage temp.

-

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

Application

Fluorescein isothiocyanate – Dextran 500000-Conjugate is used as a fluorescent tissue permeability assay reagent and as a diffusion probe for evaluation of biomaterials such as hydrogels and lipid vesicles.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Ayesha Chaudhary et al.
Biotechnology and bioengineering, 104(6), 1075-1085 (2009-08-06)
The feasibility of dissolved-core alginate-templated fluorescent microspheres as "smart tattoo" glucose biosensors was investigated in simulated interstitial fluid (SIF). The sensor works on the principle of competitive binding and fluorescence resonance energy transfer. The sensor consists of multilayer thin film
Joel Hagman et al.
Biomacromolecules, 11(12), 3359-3366 (2010-11-09)
The time-dependent diffusion and mechanical properties of gelatin in solution, in the gel state, and during the sol/gel transition were determined using fluorescence recovery after photobleaching (FRAP) and rheology. The parameters in the experimental design were 2% w/w and 5%
Tina Vermonden et al.
Macromolecules, 43(2), 782-789 (2010-10-05)
Hydrogel formation triggered by a change in temperature is an attractive mechanism for in situ gelling biomaterials for pharmaceutical applications such as the delivery of therapeutic proteins. In this study, hydrogels were prepared from ABA triblock polymers having thermosensitive poly(N-(2-hydroxypropyl)
Lisa M Dominak et al.
Langmuir : the ACS journal of surfaces and colloids, 24(23), 13565-13571 (2008-11-05)
We report the effect of macromolecular crowding on encapsulation efficiency of fluorescently labeled poly(ethylene glycol) (PEG) and dextran polymers within individual giant lipid vesicles (GVs). Low concentrations of the fluorescently labeled polymers (82 nM to 186 pM) were mixed with
Woo Jin Kim et al.
ACS nano, 14(10), 13217-13231 (2020-09-25)
Male infertility is a multifactorial condition. Unexplained male infertility is often caused by spermatogenesis dysfunction. Knockout of Pin1, an important regulator of cell proliferation and differentiation, produces male infertility phenotypes such as testicular immaturity and azoospermia with spermatogonia depletion and

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