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75917

Millipore

Brain Heart Infusion, Porcine

suitable for microbiology

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Synonym(s):
BHI Broth, Brain Heart Broth
NACRES:
NA.85

biological source

Porcine heart

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

composition

amino-nitrogen, ≥4.5%
total nitrogen (N), ≥11%

packaging

pkg of 500 g

storage condition

(Tightly closed. Dry.)

ign. residue

≤17%

loss

≤6.0% loss on drying

color

light yellow

pH

6.5-7.5 (25 °C, 2% in H2O)

solubility

H2O: 2%, clear, light yellow (and light brown-yellow and light brown)

application(s)

food and beverages
microbiology

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Brain Heart Infusion, Porcine suitable for microbiology

75917

Brain Heart Infusion, Porcine

Millipore

82962

Peptone from meat, enzymatic digest

Peptone from meat, peptic digest suitable for microbiology

70174

Peptone from meat, peptic digest

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

application(s)

food and beverages, microbiology

application(s)

food and beverages, microbiology

application(s)

food and beverages
microbiology

application(s)

food and beverages, microbiology

solubility

H2O: 2%, clear, light yellow (and light brown-yellow and light brown)

solubility

H2O: 2%, clear, yellow (and faint yellow and faint brown)

solubility

H2O: 2%, clear, faintly to light yellow

solubility

H2O: 2%, clear, slightly yellow to brown

form

powder

form

powder

form

powder

form

powder

pH

6.5-7.5 (25 °C, 2% in H2O)

pH

6.5-7.5 (2% in H2O)

pH

5.5±0.5 (25 °C, 2% in H2O)

pH

7.0±0.5 (25 °C, 2% in H2O)

General description

Brain Heart Infusion (BHI), Porcine, is a culture medium commonly used in microbiology for the cultivation of various fastidious, pathogenic microorganisms, including yeasts and molds. The media is nutritious and well-buffered to support the growth of a wide range of microorganisms such as Streptococci, Pneumococci, Meningococci, etc. The media is especially suited for the cultivation of Staphylococci for the plasma coagulase test and setting up blood cultures.BHI is typically prepared using porcine (pig) brain and heart tissues as the source of the raw materials. The enzymatic digestion of porcine brain-heart infusion provides amino acids, nitrogen, carbon, vitamins, and minerals for the growth of organisms. Glucose is the source of carbohydrates, and sodium chloride maintains the osmotic balance of the medium. Disodium phosphate is a buffering agent.

Application

Brain Heart Infusion, porcine, is recommended for the cultivation of fastidious pathogenic microorganisms and is often used for the examination of water, wastewater, meat, and food products. It is used as a culture medium for the growth of Streptococcus pneumonia, Listeria monocytogenes, Leishmania promastigotes, and Clostridium difficile.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Articles

Culture media provides a habitat with suitable nutrients, energy sources, and certain environmental conditions for the growth of microorganisms. The components of the culture media range from simple sugars to peptones, salts, antibiotics, and complex indicators.

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