B2883

bisBenzimide H 33258

≥98% (HPLC and TLC)

• Synonym(s): 2′-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi(1H-benzimidazole) trihydrochloride, 2-[2-(4-Hydroxyphenyl)-6-benzimidazoyl]-6-(1-methyl-4-piperazyl)benzimidazole trihydrochloride, BBIH, BXI-72, HOE 33258, Hoechst 33258, NSC334072
• Empirical Formula (Hill Notation): C₂₅H₂₄N₆O · 3HCl
• CAS Number: 23491-45-4
• Molecular Weight: 533.88
• Beilstein/REAXYS Number: 4088183
• EC Number: 245-690-6
• MDL number: MFCD00012679
• UNSPSC Code: 12171500
• PubChem Substance ID: 24891414
• NACRES: NA.47

Properties

• Quality Level: 200
• assay: ≥98% (HPLC and TLC)
• form: powder
• mp: 280 °C
• solubility: H₂O: 10 mg/mL; water and ethanol: 10 mg/mL; phosphate buffer: precipitates
• suitability: passes application test for fluorescence
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: −20°C
• SMILES string: Cl[H].Cl[H].Cl[H].[H]O[H].CN1CCN(CC1)c2ccc3NC(=NCc3c2)c4ccc5NC(=NCc5c4)c6ccc(O)cc6
• InChI: 1S/C27H28N6O.3ClH.H2O/c1-32-10-12-33(13-11-32)22-5-9-25-21(15-22)17-29-27(31-25)19-4-8-24-20(14-19)16-28-26(30-24)18-2-6-23(34)7-3-18;;;;/h2-9,14-15,34H,10-13,16-17H2,1H3,(H,28,30)(H,29,31);3*1H;1H2
• InChI key: OWRSPPSBNWJJAR-UHFFFAOYSA-N

Description

Application

bisBenzimide H 33258 is useful for staining DNA, chromosomes and nuclei. bisBenzimide H 33258 may be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm

bisBenzimide H 33258 has been used for nuclear staining in cells.

Biochem/physiol Actions

bisBenzimide H 33258 is a potent and selective Bcl-XL inhibitor. bisBenzimide H 33258 is a membrane-permeable, fluorescent DNA stain with low cytotoxicity that intercalate in A-T regions of DNA.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves