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E4382

Sigma-Aldrich

Eosin Y disodium salt

Dye content, ≥90%, certified by the Biological Stain Commission, powder

Synonym(s):

2′,4′,5′,7′-Tetrabromofluorescein disodium salt, Acid Red 87, Bromo acid J. TS, XL, or XX, Bromofluorescein, Bronze Bromo ES, D&C;Red No. 22, Eosin yellowish

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About This Item

Empirical Formula (Hill Notation):
C20H6Br4Na2O5
CAS Number:
17372-87-1
Molecular Weight:
691.85
Colour Index Number:
45380
Beilstein/REAXYS Number:
3586809
EC Number:
241-409-6
MDL number:
MFCD00005040
UNSPSC Code:
12171500
PubChem Substance ID:
24894551
NACRES:
NA.47

Product Name

Eosin Y disodium salt, certified by the BSC

agency

certified by the BSC

Quality Level

200

form

powder

composition

Dye content, ≥90%

technique(s)

titration: suitable

color

brown

pH

6.55 (29 °C, 84 °F)

mp

255 — 270 °C (491 - 518 °F at 977.9 hPa)

solubility

H2O: 1 mg/mL

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Application

Eosin Y disodium salt has been used as a counterstain in immunocytochemistry and immunohistochemistry.[1][2]

Biochem/physiol Actions

Eosin Y is a xanthene dye and is used for the differential staining of connective tissue and cytoplasm. In histopathology, it is applied as a counterstain after hematoxylin and before methylene blue. It is also used as a background stain, thereby giving contrast to the nuclear stains.[3][4]

Suitability

Certified for use as a counterstain against hematoxylin; as a constituent of Wright stain; in modified Nocht′s stain; as a constituent in eosin-methylene blue medium.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317,H319

Hazard Classifications

Eye Irrit. 2 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

376.9 °F - Pensky-Martens closed cup

flash_point_c

191.6 °C - Pensky-Martens closed cup

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000421986
0000421986
SHBS3896
0000410611
0000368238

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Dendritic cell loss from nonlymphoid tissues after systemic administration of lipopolysaccharide, tumor necrosis factor, and interleukin 1.
Roake JA, et al.
The Journal of Experimental Medicine, 181, 2237-2237 (1995)
Different expression of occludin and ZO-1 in primary and metastatic liver tumors.
Orban E, et al.
Pathology Oncology Research, 14, 299-299 (2008)
Bancroft JD
Theory and Practice of Histological Techniques (2008)
Joanne M Donkers et al.
Cellular and molecular gastroenterology and hepatology, 10(3), 451-466 (2020-04-25)
Bile acids are important metabolic signaling molecules. Bile acid receptor activation promotes body weight loss and improves glycemic control. The incretin hormone GLP-1 and thyroid hormone activation of T4 to T3 have been suggested as important contributors. Here, we identify
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