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N2125

Sigma-Aldrich

Naphthol AS-BI phosphate

Technical grade, ≥98% (HPLC)

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Synonym(s):
7-Bromo-3-hydroxy-2-naphthoic-o-anisidide phosphate
Empirical Formula (Hill Notation):
C18H15BrNO6P
CAS Number:
Molecular Weight:
452.19
Beilstein/REAXYS Number:
6668526
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

assay

≥98% (HPLC)

form

powder

color

white to light yellow

solubility

methanol: 50 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

COc1ccccc1NC(=O)c2cc3cc(Br)ccc3cc2OP(O)(O)=O

InChI

1S/C18H15BrNO6P/c1-25-16-5-3-2-4-15(16)20-18(21)14-9-12-8-13(19)7-6-11(12)10-17(14)26-27(22,23)24/h2-10H,1H3,(H,20,21)(H2,22,23,24)

InChI key

HUXIAXQSTATULQ-UHFFFAOYSA-N

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General description

Naphthol AS-BI, also known as 7-Bromo-3-hydroxy-2-naphtho-o-anisidine is a substituted naphthol.

Application

Naphthol AS-BI phosphate is suitable to use:
  • in tartrate-resistant acid phosphatase (TRAP) staining to detect osteoclast activity
  • as a component in Tris-HCl in staining cells to measure alkaline phosphatase (ALP) activity
  • to perform alkaline phosphatase reaction
Substrate for the histochemical demonstration of acid and alkaline phosphatase. Substrate for the fluorometric assay of acid and alkaline phosphatases. Demonstration of acid phosphatase in paraffin embedded tissue. Cytofluorometric assay of acid phosphatase in single living cells.

Biochem/physiol Actions

Naphthol AS-BI can be used as a substrate to determine alkaline and acid phosphatase. It is also used to determine the activity of acid phosphatase in single cells.

Substrates

Substrate for the histochemical demonstration of acid and alkaline phosphatase.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


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