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F0500

Sigma-Aldrich

Fast Blue RR Salt

crystalline

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Synonym(s):
4-Benzoylamino-2,5-dimethoxybenzenediazonium chloride hemi(zinc chloride) salt, Azoic Diazo No. 24
Linear Formula:
C15H14ClN3O3 · 1/2 ZnCl2
CAS Number:
Molecular Weight:
387.89
Colour Index Number:
37155
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

form

crystalline

Quality Level

solubility

water: 1 mg/mL, clear to hazy, yellow-green

ε (extinction coefficient)

≥15000 at 326-332  nm
≥7000 at 390-396 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.COc1cc([N+]#N)c(OC)cc1NC(=O)c2ccccc2.COc3cc([N+]#N)c(OC)cc3NC(=O)c4ccccc4

InChI

1S/2C15H13N3O3.4ClH.Zn/c2*1-20-13-9-12(18-16)14(21-2)8-11(13)17-15(19)10-6-4-3-5-7-10;;;;;/h2*3-9H,1-2H3;4*1H;/q;;;;;;+2/p-2

InChI key

LLARVKAOQLUCQI-UHFFFAOYSA-L

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1 of 4

This Item
F3378F2256FBS25
Fast Blue RR Salt crystalline

F0500

Fast Blue RR Salt

Fast Red RC Salt

F2256

Fast Red RC Salt

Fast Blue RR Salt Preweighed Capsules

FBS25

Fast Blue RR Salt

solubility

water: 1 mg/mL, clear to hazy, yellow-green

solubility

H2O: 10 mg/mL

solubility

water: 1 mg/mL

solubility

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

hematology
histology

storage temp.

−20°C

storage temp.

−20°C

storage temp.

room temp

storage temp.

−20°C

εmax

≥15000 at 326-332  nm

εmax

-

εmax

-

εmax

-

Application

Fast blue RR salt has been used in the immunohistochemistry to detect the activity of alkaline phosphatase. It has been used in the procedure of connective tissue staining using picrosirius red.

Biochem/physiol Actions

Fast blue RR is mainly used in histological stainings. It binds to the reaction product of alkaline phosphatase and precipitates it as an insoluble compound. The sites of alkaline phosphatase activity appears black. Fast blue RR is also used for the detection of esterase activity. It couples with naphthol (released from naphthol acetate) and forms an insoluble azo-dye which appears red/blue.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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