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D9805

Sigma-Aldrich

Fast Blue B Salt

Dye content ~95 %

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Synonym(s):
o-Dianisidine bis(diazotized) zinc double salt, Azoic Diazo No. 48, DBB, Diazo Blue B, Naphthanil Diazo Blue B
Linear Formula:
C14H12N4O2Cl2 · ZnCl2
CAS Number:
Molecular Weight:
475.47
Colour Index Number:
37235
Beilstein/REAXYS Number:
1499067
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

description

Technical grade

Quality Level

form

powder

composition

Dye content, ~95%

color

yellow to dark brown

mp

>300 °C (lit.)

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.COc1cc(ccc1[N+]#N)-c2ccc([N+]#N)c(OC)c2

InChI

1S/C14H12N4O2.4ClH.Zn/c1-19-13-7-9(3-5-11(13)17-15)10-4-6-12(18-16)14(8-10)20-2;;;;;/h3-8H,1-2H3;4*1H;/q+2;;;;;+2/p-4

InChI key

GPPKNJIWDULNQH-UHFFFAOYSA-J

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1 of 4

This Item
F0500F0125F0750
vibrant-m

D9805

Fast Blue B Salt

vibrant-m

F0500

Fast Blue RR Salt

vibrant-m

F0750

Fast Dark Blue R Salt

form

powder

form

crystalline

form

powder

form

solid

mp

>300 °C (lit.)

mp

-

mp

98-100 °C

mp

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

room temp

storage temp.

−20°C

composition

Dye content, ~95%

composition

-

composition

Dye content, ~95%

composition

Dye content, ~20%

Application

  • Fast Blue B has been used in the acid phosphatase test in Clostridium perfringens.
  • It has been used as a spraying agent in thin layer chromatography for the identification of separated components.
  • It has also been used for the determination of the lipase activity.

Biochem/physiol Actions

Fast blue B is used for the determination of acetylcholinesterase activity. Acetylcholinesterase converts 1-naphthyl acetate to 1-naphthol, which reacts with fast blue B to form a purple-coloured diazonium dye. Similarly, fast blue B is also used for α- and β-glucosidase activity.

pictograms

FlameHealth hazard

signalword

Danger

Hazard Classifications

Carc. 1B - Self-react. C

Storage Class

5.2 - Organic peroxides and self-reacting hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges


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In this study, composite anion exchange membranes (AEMs) were synthesized by cross-linking poly(phenylene oxide) (PPO) with cellulose functionalized by 1,4-diazabicyclo[2.2.2]-octane (DABCO) or di-guanidine (DG). The structural and morphological characteristics of the synthesized AEMs were characterized by FTIR, 1H-NMR, SEM, TEM
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A TLC bioautographic method for the detection of alpha- and beta-glucosidase inhibitors in plant extracts.
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C N Kennett et al.
Journal of clinical periodontology, 24(6), 424-431 (1997-06-01)
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