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F7253

Sigma-Aldrich

Fast Black K Salt hemi(zinc chloride) salt

practical grade

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Synonym(s):
2,5-Dimethoxy-4-([4-nitrophenyl]azo)benzenediazonium chloride hemi(zinc chloride) salt, Brentamine fast black K salt
Linear Formula:
C14H12N5O4 · 1/2ZnCl4
CAS Number:
Molecular Weight:
417.88
Colour Index Number:
37190
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

grade

practical grade

Quality Level

form

powder

composition

Dye content, ≥25%

color

brown-red

mp

150 °C

solubility

water: 10 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.COc1cc([N+]#N)c(OC)cc1N=Nc2ccc(cc2)[N+]([O-])=O.COc3cc([N+]#N)c(OC)cc3N=Nc4ccc(cc4)[N+]([O-])=O

InChI

1S/2C14H12N5O4.4ClH.Zn/c2*1-22-13-8-12(14(23-2)7-11(13)16-15)18-17-9-3-5-10(6-4-9)19(20)21;;;;;/h2*3-8H,1-2H3;4*1H;/q2*+1;;;;;+2/p-4

InChI key

NZWZSUSPTZUGNN-UHFFFAOYSA-J

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F2256

Fast Red RC Salt

Fast Red TR Salt Dye content 15 %

368881

Fast Red TR Salt

solubility

water: 10 mg/mL

solubility

H2O: 10 mg/mL

solubility

water: 1 mg/mL

solubility

-

form

powder

form

powder

form

powder

form

powder

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

storage temp.

−20°C

storage temp.

room temp

storage temp.

room temp

composition

Dye content, ≥25%

composition

Dye content, ≥80%

composition

Dye content, ~20%

composition

Dye content, 15%

General description

Fast black K (C.l. 37190) is a lipophilic amino-substituted monoazo dye. It is also known as Brentamine fast black K salt.

Application

Fast Black K Salt hemi (zinc chloride) salt has been used for the staining of acid phosphatase activity in SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and native-PAGE. It has been used for staining endopeptidase and leucine aminopeptidase activity.

Fast black K salt has various applications as listed below-

  • to stain enterochromaffin cells > as an indirect stain for periodate-generated tissue aldehyde groups
  • as a visualization agent for amphetamines and cyanobacterial toxins following their separation by thin-layer chromatography
  • for the staining of acid phosphatase activity in SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and native-PAGE
  • staining endopeptidase and leucine aminopeptidase activity

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


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