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MilliporeSigma

F7253

Sigma-Aldrich

Fast Black K Salt hemi(zinc chloride) salt

Dye content, ≥25%, practical grade, powder

Synonym(s):

2,5-Dimethoxy-4-([4-nitrophenyl]azo)benzenediazonium chloride hemi(zinc chloride) salt, Brentamine fast black K salt

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About This Item

Linear Formula:
C14H12N5O4 · 1/2ZnCl4
CAS Number:
Molecular Weight:
417.88
Colour Index Number:
37190
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

Product Name

Fast Black K Salt hemi(zinc chloride) salt, practical grade

grade

practical grade

Quality Level

form

powder

composition

Dye content, ≥25%

technique(s)

microbe id | staining: suitable

color

brown-red

mp

150 °C

solubility

water: 10 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

General description

Fast black K (C.l. 37190) is a lipophilic amino-substituted monoazo dye. It is also known as Brentamine fast black K salt.

Application

Fast Black K Salt hemi (zinc chloride) salt has been used for the staining of acid phosphatase activity in SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and native-PAGE.[1] It has been used for staining endopeptidase and leucine aminopeptidase activity.[2]

Fast black K salt has various applications as listed below-

  • to stain enterochromaffin cells > as an indirect stain for periodate-generated tissue aldehyde groups
  • as a visualization agent for amphetamines and cyanobacterial toxins following their separation by thin-layer chromatography
  • for the staining of acid phosphatase activity in SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and native-PAGE
  • staining endopeptidase and leucine aminopeptidase activity

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


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Genetic control of isozymes in the primary gene pool Phaseolus lunatus L.
Zoro BI
Biotechnologie, agronomie, societe et environnement, 3, 10-27 (1999)
Analyses of Root-secreted Acid Phosphatase Activity in Arabidopsis.
Wang L and Liu D
Bio-protocol null

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