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D3252

Sigma-Aldrich

o-Dianisidine dihydrochloride

≥95%

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Synonym(s):
3,3′-Dimethoxybenzidine dihydrochloride, Fast Blue B
Empirical Formula (Hill Notation):
C14H16N2O2 · 2HCl
CAS Number:
Molecular Weight:
317.21
Beilstein/REAXYS Number:
3917996
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

assay

≥95%

form

powder

mp

268 °C

solubility

H2O: soluble 25 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

Cl.Cl.COc1cc(ccc1N)-c2ccc(N)c(OC)c2

InChI

1S/C14H16N2O2.2ClH/c1-17-13-7-9(3-5-11(13)15)10-4-6-12(16)14(8-10)18-2;;/h3-8H,15-16H2,1-2H3;2*1H

InChI key

UXTIAFYTYOEQHV-UHFFFAOYSA-N

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This Item
D9143D9154F5803
o-Dianisidine dihydrochloride ≥95%

D3252

o-Dianisidine dihydrochloride

o-Dianisidine peroxidase substrate

D9143

o-Dianisidine

o-Dianisidine dihydrochloride tablet, 10 mg substrate per tablet

D9154

o-Dianisidine dihydrochloride

o-Dianisidine dihydrochloride Suitable for use in glucose determination

F5803

o-Dianisidine dihydrochloride

solubility

H2O: soluble 25 mg/mL

solubility

H2O: slightly soluble, aqueous acid: soluble

solubility

water: 1 tablet/10 mL, clear, colorless

solubility

water: 2.5 mg/mL, colorless

Quality Level

200

Quality Level

-

Quality Level

-

Quality Level

-

form

powder

form

powder

form

tablet

form

crystalline

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

storage temp.

2-8°C

mp

268 °C

mp

-

mp

-

mp

268 °C

Specificity

o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.

Application

o-Dianisidine (3,3′-dimethoxybenzidine) has been used as a component of GO (glucose oxidase) reagent, which is used for measuring the glucose content. It has also been used as a substrate to measure myeloperoxidase activity.

Warning

Probably carcinogenic.

Physical form

crystalline

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Skin Corr. 1

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges


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