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F5803

Sigma-Aldrich

o-Dianisidine dihydrochloride

Suitable for use in glucose determination

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Synonym(s):
3,3′-Dimethoxybenzidine dihydrochloride, Fast Blue B
Empirical Formula (Hill Notation):
C14H16N2O2 · 2HCl
CAS Number:
Molecular Weight:
317.21
Beilstein/REAXYS Number:
3917996
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

form

crystalline

pH range

6.95-7.05

mp

268 °C

solubility

water: 2.5 mg/mL, colorless

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

Cl.Cl.COc1cc(ccc1N)-c2ccc(N)c(OC)c2

InChI

1S/C14H16N2O2.2ClH/c1-17-13-7-9(3-5-11(13)15)10-4-6-12(16)14(8-10)18-2;;/h3-8H,15-16H2,1-2H3;2*1H

InChI key

UXTIAFYTYOEQHV-UHFFFAOYSA-N

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This Item
D3252D9154D9143
o-Dianisidine dihydrochloride Suitable for use in glucose determination

F5803

o-Dianisidine dihydrochloride

o-Dianisidine dihydrochloride ≥95%

D3252

o-Dianisidine dihydrochloride

o-Dianisidine dihydrochloride tablet, 10 mg substrate per tablet

D9154

o-Dianisidine dihydrochloride

o-Dianisidine peroxidase substrate

D9143

o-Dianisidine

form

crystalline

form

powder

form

tablet

form

powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

pH range

6.95-7.05

pH range

-

pH range

-

pH range

-

mp

268 °C

mp

268 °C

mp

-

mp

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

-

General description

A preweighed vial containing 50 mg o-Dianisidine.

Specificity

o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.

Application

o-Dianisidine dihydrochloride is suitable for use in the glucose determination. It is suitable reagent used in the following studies:
  • As substrate for the determination of serum ceruloplasmin from its oxidase activity.
  • Determination of total antioxidant response (TAR) against potent free radical reactions by a novel, colorimetric and fully automated method.
  • As colorimetric indicator for the simultaneous semi-quantitative estimation of glucose, lactate and uric acid on paper-based microfluidic devices (microPAD).
  • Myeloperoxidase activity assay.

Warning

Probably carcinogenic.

enzyme

Product No.
Description
Pricing

standard

Product No.
Description
Pricing

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Skin Corr. 1

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges


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Measurement of ceruloplasmin from its oxidase activity in serum by use of o-dianisidine dihydrochloride.
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We report here the use of multiple indicators for a single analyte for paper-based microfluidic devices (microPAD) in an effort to improve the ability to visually discriminate between analyte concentrations. In existing microPADs, a single dye system is used for

Protocols

To standardize a procedure for the enzymatic assay of Diamine Oxidase.

Glucose oxidase activity measured via continuous spectrophotometric assay at 500 nm, indicating glucose oxidation rate.

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