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T8778

Sigma-Aldrich

Trehalase from porcine kidney

buffered aqueous glycerol solution, ≥1.0 units/mg protein

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Synonym(s):
α,α-Trehalose glucohydrolase
CAS Number:
Enzyme Commission number:
MDL number:
NACRES:
NA.54

form

buffered aqueous glycerol solution

specific activity

≥1.0 units/mg protein

concentration

0.5-10.0 mg/mL protein (BCA)

storage temp.

−20°C

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This Item
T016790210T9449
D-(+)-Trehalose dihydrate suitable for microbiology, ≥99.0%, composed of two α-glucose units that is used as a protectant, stabilizer and to support proper folding

Millipore

90210

D-(+)-Trehalose dihydrate

specific activity

≥1.0 units/mg protein

specific activity

-

specific activity

-

specific activity

-

concentration

0.5-10.0 mg/mL protein (BCA)

concentration

-

concentration

-

concentration

-

storage temp.

−20°C

storage temp.

-

storage temp.

-

storage temp.

room temp

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-

General description

Trehalase is a glycosidase that occurs in many forms and is found in all tissues. It belongs to the glycoside hydrolase 37 (GH37) family of the carbohydrate-active enzyme (CAZy) classification.
Trehalose is the main haemolymph sugar in most insects and is potentially a prime target for an invading pathogenic fungus.

Application

Trehalase has been used:
  • in a study to assess changes in carbohydrate metabolism in Plasmopara viticola-infected grapevine leaves
  • in a study to investigate growth arrest by trehalose-6-phosphate
  • to quantify the intracellular trehalose in Lactobacillus casei cells

Biochem/physiol Actions

Trehalase catalyzes the hydrolysis of trehalose to produce two glucose units. It mediates flight metabolism, molting induced chitin production and tolerance towards cold temperature.

Unit Definition

One unit will convert 1.0 μmole of trehalose to 2.0 μmoles of glucose per min at pH 5.7 at 37 °C (liberated glucose determined at pH 7.5).

Physical form

Solution in 50% glycerol containing 1% Triton X-100 and 25 mM potassium phosphate, pH 6.5

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Jiangnan Luo et al.
PloS one, 9(6), e99732-e99732 (2014-06-14)
A set of 14 insulin-producing cells (IPCs) in the Drosophila brain produces three insulin-like peptides (DILP2, 3 and 5). Activity in IPCs and release of DILPs is nutrient dependent and controlled by multiple factors such as fat body-derived proteins, neurotransmitters
Christopher Askew et al.
PLoS pathogens, 5(10), e1000612-e1000612 (2009-10-10)
Glycolysis is a metabolic pathway that is central to the assimilation of carbon for either respiration or fermentation and therefore is critical for the growth of all organisms. Consequently, glycolytic transcriptional regulation is important for the metabolic flexibility of pathogens
Yu Sasano et al.
Journal of bioscience and bioengineering, 113(5), 592-595 (2012-01-28)
Freeze tolerance is a necessary characteristic for industrial baker's yeast because frozen-dough baking is one of the key technologies for supplying oven-fresh bakery products to consumers. Both proline and trehalose are known to function as cryoprotectants in yeast cells. In
Trehalose-hydrolysing enzymes of Metarhizium anisopliae and their role in pathogenesis of the tobacco hornworm, Manduca sexta
Xia Y, Clarkson JM, Charnley AK.
J. Invert. Path., 80(3), 139-147 (2012)
Jie Chen et al.
PloS one, 5(4), e10133-e10133 (2010-04-21)
Trehalase, an enzyme that hydrolyzes trehalose to yield two glucose molecules, plays a pivotal role in various physiological processes. In recent years, trehalase proteins have been purified from several insect species and are divided into soluble (Tre-1) and membrane-bound (Tre-2)

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