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201677

Sigma-Aldrich

Janus Green B

certified by the Biological Stain Commission, Dye content 65 %

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Synonym(s):
3-Diethylamino-7-(4-dimethylaminophenylazo)-5-phenylphenazinium chloride, Diazin Green S, Union Green B
Empirical Formula (Hill Notation):
C30H31ClN6
CAS Number:
Molecular Weight:
511.06
Colour Index Number:
11050
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

form

powder

composition

Dye content, 65%

λmax

395 nm
660 nm (2nd)

ε (extinction coefficient)

≥28000 at 651-677 nm in ethanol: water (1:1) at 0.01 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[Cl-].CCN(CC)c1ccc2nc3ccc(cc3[n+](-c4ccccc4)c2c1)\N=N\c5ccc(cc5)N(C)C

InChI

1S/C30H31N6.ClH/c1-5-35(6-2)26-17-19-28-30(21-26)36(25-10-8-7-9-11-25)29-20-23(14-18-27(29)31-28)33-32-22-12-15-24(16-13-22)34(3)4;/h7-21H,5-6H2,1-4H3;1H/q+1;/p-1

InChI key

XXACTDWGHQXLGW-UHFFFAOYSA-M

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1 of 4

This Item
B6756B4014199583
Janus Green B certified by the Biological Stain Commission, Dye content 65 %

Sigma-Aldrich

201677

Janus Green B

Brilliant Green Dye content ~90 %

Sigma-Aldrich

B6756

Brilliant Green

Brilliant Green certified by the Biological Stain Commission

Sigma-Aldrich

B4014

Brilliant Green

Lissamine™ Green B Dye content 60 %

Sigma-Aldrich

199583

Lissamine Green B

form

powder

form

powder

form

powder

form

powder

composition

Dye content, 65%

composition

Dye content, ~90%

composition

Dye content, ≥90%

composition

Dye content, 60%

λmax

395 nm, 660 nm (2nd)

λmax

-

λmax

-

λmax

633 nm

ε (extinction coefficient)

≥28000 at 651-677 nm in ethanol: water (1:1) at 0.01 g/L

ε (extinction coefficient)

-

ε (extinction coefficient)

-

ε (extinction coefficient)

≥10000 at 300-306 nm in water at 0.005 g/L, ≥50000 at 236-242 nm in water at 0.005 g/L, ≥60000 at 630-636 nm in water at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

Application

Janus Green B has been used in staining technique to determine cell density, to detect the presence of telocytes in trachea and lungs and used as a dye to visualize bonghan ducts inside lymphatic vessels of rabbits.

Biochem/physiol Actions

Janus Green B belongs to the phenazine group of dyes. It is a dark green/dark brown/dark black powder. Janus Green B should be oxidized to become colored. Thus, this dye can be used to specifically stain mitochondria in living cells. It has been used to stain biomolecules, nucleic acids and chromosomes. In addition, it is also used to stain various living organisms including fungi, yeast cell, brain, spinal cord, sperms and tissue culture monolayer. Janus Green B acts as an antimalarial agent. It also facilitates diagnostic assays and diagnosis of disease related to amyloid accumulation.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Customers Also Viewed

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David E. Sadava
Cell Biology: Organelle Structure and Function (1993)
Intracellular insulin quantification by cell-ELISA
Andersen P and Vermette P
Experimental Cell Research, 347, 14-23 (2016)
R. W. Sabnis
Handbook of Biological Dyes and Stains: Synthesis and Industrial Applications (2010)
Novel threadlike structures (Bonghan ducts) inside lymphatic vessels of rabbits visualized with a Janus Green B staining method
Lee BC
Anatomical Record. Part B, New Anatomist, 286, 1-7 (2005)
Telocytes in trachea and lungs
Zheng Y
Journal of Cellular and Molecular Medicine, 15, 2262-2268 (2011)

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