Skip to Content
MilliporeSigma
All Photos(4)

Key Documents

View All Documentation

P5504

Sigma-Aldrich

Potassium phosphate dibasic trihydrate

ReagentPlus®, ≥99.0%

Synonym(s):

Dipotassium hydrogen phosphate trihydrate, Dipotassium phosphate, di-Potassium hydrogen phosphate trihydrate

Sign Into View Organizational & Contract Pricing
All Photos(4)

About This Item

Linear Formula:
K2HPO4 · 3H2O
CAS Number:
16788-57-1
Molecular Weight:
228.22
EC Number:
231-834-5
MDL number:
MFCD00149926
UNSPSC Code:
12161700
PubChem Substance ID:
57654537
NACRES:
NA.25

Quality Level

200

product line

ReagentPlus®

assay

≥99.0%

form

powder or crystals

pH

9.2-9.4 (25 °C, 50 g/L)

solubility

water: 100 mg/mL, clear, colorless

storage temp.

room temp

SMILES string

O.O.O.[K+].[K+].OP([O-])([O-])=O

InChI

1S/2K.H3O4P.3H2O/c;;1-5(2,3)4;;;/h;;(H3,1,2,3,4);3*1H2/q2*+1;;;;/p-2

InChI key

XQGPKZUNMMFTAL-UHFFFAOYSA-L

Looking for similar products? Visit Product Comparison Guide

General description

Potassium phosphate solution have very high buffering capacity that is widely used in molecular biology, biochemistry, and chromatography. Potassium phosphate dibasic trihydrate is a key component of potassium phosphate buffer, phosphate-buffered saline (PBS), and phosphate-buffered EDTA (PBE). It is extensively used in the preparation of biological samples for various biological experiments. Potassium phosphate buffer is also used in methods such as equilibrium dialysis, high-performance liquid chromatography (HPLC), and affinity capillary electrophoresis (ACE) to study the characteristics of drug-protein interactions in the body. Potassium phosphate buffer solutions consist of mixtures of the potassium phosphate monobasic and potassium phosphate dibasic forms.

Application

Potassium phosphate dibasic trihydrate has been used in the preparation of potassium phosphate buffer for the resuspension of polyelectrolyte capsules. It has also been used as a buffer during glutaminase‑1 (GLS-1) enzyme assay and GLS-2 enzyme assay.

Legal Information

ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000398339
0000398858
0000399362
0000398859
0000394551

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Interaction between Potassium Phosphate Buffer Solution and Modeling Cell Membrane Investigated by Sum Frequency Generation Vibrational Spectroscopy
Weilai Y, et al.
Chinese Journal of Chemical Physics, 28(4), 518-524 (2015)
Daniel Valdepérez et al.
Journal of colloid and interface science, 474, 1-8 (2016-04-19)
Polyelectrolyte hollow capsules are versatile platforms typically used for encapsulation of a wide variety of macromolecules in their cavity. The polymer shell of these capsules as composed by alternating layers of oppositely charged polyelectrolytes also allows for adding additional functionalities.
Imidazolium-based Ionic Liquids as Adjuvants to Form Polyethylene Glycol with Salt Buffer Aqueous Biphasic Systems
Santos JHPM, et al.
Journal of Chemical and Engineering Data, 65(8), 3794?3801-3794?3801 (2020)
Antoine Desrut et al.
Journal of experimental botany, 71(22), 7301-7315 (2020-08-30)
Plants live in close relationships with complex populations of microorganisms, including rhizobacterial species commonly referred to as plant growth-promoting rhizobacteria (PGPR). PGPR are able to improve plant productivity, but the molecular mechanisms involved in this process remain largely unknown. Using
Michael J Soth et al.
Journal of medicinal chemistry, 63(21), 12957-12977 (2020-10-30)
Inhibition of glutaminase-1 (GLS-1) hampers the proliferation of tumor cells reliant on glutamine. Known glutaminase inhibitors have potential limitations, and in vivo exposures are potentially limited due to poor physicochemical properties. We initiated a GLS-1 inhibitor discovery program focused on
View All Related Papers

Protocols

Enzymatic Assay of Superoxide Dismutase

Enzymatic Assay of Superoxide Dismutase

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service