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08715

Sigma-Aldrich

DAR-2

BioReagent, suitable for fluorescence, ≥97.0% (HPCE)

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Synonym(s):
5,6-Diamino-N,N,N′,N′-tetraethylrhodamine, 5,6-Diamino-rhodamine B
Empirical Formula (Hill Notation):
C28H32N4O3
CAS Number:
Molecular Weight:
472.58
MDL number:
PubChem Substance ID:
NACRES:
NA.32

product line

BioReagent

Quality Level

assay

≥97.0% (HPCE)

form

powder

fluorescence

λex 562 nm; λem 579 nm in 0.1 M phosphate pH 7.4, NO

suitability

suitable for fluorescence

SMILES string

CCN(CC)c1ccc2c(Oc3cc(ccc3C24OC(=O)c5cc(N)c(N)cc45)N(CC)CC)c1

InChI

1S/C28H32N4O3/c1-5-31(6-2)17-9-11-20-25(13-17)34-26-14-18(32(7-3)8-4)10-12-21(26)28(20)22-16-24(30)23(29)15-19(22)27(33)35-28/h9-16H,5-8,29-30H2,1-4H3

InChI key

LJSMMWFTVBPRDS-UHFFFAOYSA-N

Application

Suitable as fluorescent NO-sensor.

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H Kojima et al.
Analytical chemistry, 73(9), 1967-1973 (2001-05-17)
Diaminofluoresceins are widely used for detection and imaging of nitric oxide (NO), but for biological applications, they have the disadvantages that the fluorescence of the fluorescein chromophore is pH-sensitive and overlaps the autofluorescence of cells. We have developed a membrane-permeable
H. Kojima et al.
Tetrahedron Letters, 41, 69-69 (2000)

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