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10417

Sigma-Aldrich

8-Anilino-1-naphthalenesulfonic acid ammonium salt

for fluorescence, ≥97.0% (HPLC)

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Synonym(s):
1,8-ANS NH4, ANSA, Ammonium 8-anilino-1-naphthalenesulfonate, N-Phenyl peri acid
Linear Formula:
C16H13NO3S · NH3
CAS Number:
Molecular Weight:
316.37
Beilstein/REAXYS Number:
3581235
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.32

grade

for fluorescence

Quality Level

assay

≥97.0% (HPLC)

form

solid

loss

≤2.5% loss on drying

mp

245-253 °C

solubility

H2O: 0.1 g/5mL, clear to very slightly hazy (hot)
NaOH: 1 N
H2O: soluble
acetone: soluble
methanol: soluble

fluorescence

λex 388 nm; λem 470 nm in 0.1 M Tris, 0.2 M KCl, pH 9.0, BSA

SMILES string

N.OS(=O)(=O)c1cccc2cccc(Nc3ccccc3)c12

InChI

1S/C16H13NO3S.H3N/c18-21(19,20)15-11-5-7-12-6-4-10-14(16(12)15)17-13-8-2-1-3-9-13;/h1-11,17H,(H,18,19,20);1H3

InChI key

IPBNQYLKHUNLQE-UHFFFAOYSA-N

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1 of 4

This Item
10416A1028A7460
8-Anilino-1-naphthalenesulfonic acid

A1028

8-Anilino-1-naphthalenesulfonic acid

-
Ammonium persulfate BioXtra, ≥98.0%

A7460

Ammonium persulfate

Premium Grade
solubility

H2O: 0.1 g/5mL, clear to very slightly hazy (hot), H2O: soluble, methanol: soluble, NaOH: 1 N, acetone: soluble

solubility

NaOH: 1 N, H2O: soluble, methanol: soluble

solubility

DMF: 50 mg/mL

solubility

H2O: 0.5 M at 20 °C, clear, colorless

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

mp

245-253 °C

mp

-

mp

215-217 °C (dec.) (lit.)

mp

-

form

solid

form

powder

form

powder

form

powder

grade

for fluorescence

grade

-

grade

-

grade

-

Application

ANS forms an inclusion complex with cyclodextrin. Such model systems are useful to mimic biological recognition and can be studied by measuring the change in fluorescence of free-ANS to complexed-ANS. When ANS enters the hydrophobic core of cyclodestrin, it′s fluorescence increases . Utilized in the reagent phase of a sodium-selective fiber-optic sensor. The reagent phase also contains a copper(II) polyelectrolyte, which binds to ANSA in the absence of sodium and quenches the fluorescence. In the presence of sodium, ANSA forms a cationic complex creating ion-pairs, causing it to fluoresce . ANS is often incorporated into di-block polymers and can be released by changes in the local environment (i.e., temperature, pH, etc.) . ANS is commonly used as a fluorescence probe to investigate molecular assemblies of surfactants and amphiphilic polymers because a blue shift of the emission maximum indicates the fluorophore is located in less polar media . Fluorescent probe for protein studies using methodologies such as steady-state and dynamic fluorescence measurements .
This product is an amphiphilic fluorescent probe for protein studies . Excitation of the unbound dye at 380 nm results in a low fluorescent emission with a maximum at 545 nm. The fluorescence intensity of ANS increases when the dye binds to the hydrophobic regions of a protein . The protein-ANS complex has an emission spectrum which is shifted to a broad maximum at 470 nm. At pH 8, protein causes a 40-fold increase in the relative quantum yield compared to free ANS in solution . ANS has been used to monitor protein conformational changes by binding to the hydrophobic regions of a protein , to gain new insight into protein binding interactions, often by acting as reporter or competitor ligands, to investigate the visual excitation process and structural aspects of photoreceptor cell membranes , and to probe (and disrupt) the structure of both high- and low-density lipoproteins. It has also been used as a substrate in a chemiluminescent enzyme immunoassay system and as a dye for yeast viability determination. The conformational states for apo- and holo- yeast alcohol dehydrogenase were reported under conditions of low pH using ANS fluorescence . ANS is also commonly used as a fluorescence probe to investigate molecular assemblies of surfactants and amphiphilic polymers because a blue shift of its emission maximum indicates the probe is located in less polar environment

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


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Quantitative estimation of protein binding site polarity. Fluorescence of N-arylaminonaphthalenesulfonates.
D C Turner et al.
Biochemistry, 7(10), 3381-3390 (1968-10-01)
Evaluation of the fluorescent dye 1-anilino-8-naphtahalene sulfonic acid for yeast viability determination
McCaig, R.
Journal of the American Society of Brewing Chemists, 48, 22-22 (1990)
Oktay K Gasymov et al.
Biochemistry, 47(5), 1414-1424 (2008-01-09)
Human tear lipocalin (TL) exhibits diverse functions, most of which are linked to ligand binding. To map the binding site of TL for some amphiphilic ligands, we capitalized on the hydrophobic and hydrophilic properties of 8-anilino-1-naphthalenesulfonic acid (ANS). In single
L D'Alfonso et al.
Biochimica et biophysica acta, 1432(2), 194-202 (1999-07-17)
Steady-state and dynamic fluorescence titrations show that: (a) the complex between beta-lactoglobulin (BLG) and 1-anilinonaphthalene-8-sulfonate (ANS) displays a heterogeneous equilibrium with large changes in the binding strength vs. pH and ion concentration; and (b) the fluorescence response of bound ANS
Maddalena Collini et al.
Protein science : a publication of the Protein Society, 12(8), 1596-1603 (2003-07-24)
The use of spectroscopy in the study of fatty acids binding to bovine beta-lactoglobulin (BLG) appears to be a difficult task, as these acid compounds, assumed as the protein natural ligands, do not exhibit favorable optical response such as, for

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