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125M-1

Sigma-Aldrich

CD25 (4C9) Mouse Monoclonal Antibody

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NACRES:
NA.41

biological source

mouse

Quality Level

100
500

conjugate

unconjugated

antibody form

culture supernatant

antibody product type

primary antibodies

clone

4C9, monoclonal

description

For In Vitro Diagnostic Use in Select Regions (See Chart)

form

buffered aqueous solution

species reactivity

human

packaging

vial of 0.1 mL concentrate (125M-14)
vial of 0.5 mL concentrate (125M-15)
bottle of 1.0 mL predilute (125M-17)
vial of 1.0 mL concentrate (125M-16)
bottle of 7.0 mL predilute (125M-18)

manufacturer/tradename

Cell Marque

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:10-1:50

isotype

IgG2b

control

lesions of mastocytosis

shipped in

wet ice

storage temp.

2-8°C

visualization

cytoplasmic, membranous

Gene Information

human ... IL2RA(3559)

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This Item
123M-2120M-8123M-1
species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

clone

4C9, monoclonal

clone

MRQ-57, monoclonal

clone

L26, monoclonal

clone

1B12, monoclonal

antibody form

culture supernatant

antibody form

culture supernatant

antibody form

diluted ascites fluid

antibody form

culture supernatant

packaging

vial of 0.1 mL concentrate (125M-14), bottle of 1.0 mL predilute (125M-17), bottle of 7.0 mL predilute (125M-18), vial of 0.5 mL concentrate (125M-15), vial of 1.0 mL concentrate (125M-16)

packaging

vial of 0.1 mL concentrate (123M-24), vial of 0.5 mL concentrate (123M-25), bottle of 1.0 mL predilute (123M-27), vial of 1.0 mL concentrate (123M-26), bottle of 7.0 mL predilute (123M-28)

packaging

vial of 0.1 mL concentrate (120M-84), vial of 0.5 mL concentrate (120M-85), bottle of 1.0 mL predilute (120M-87), vial of 1.0 mL concentrate (120M-86), bottle of 7.0 mL predilute (120M-88)

packaging

vial of 0.1 mL concentrate (123M-14), vial of 0.5 mL concentrate (123M-15), bottle of 1.0 mL predilute (123M-17), vial of 1.0 mL concentrate (123M-16), bottle of 7.0 mL predilute (123M-18)

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General description

According to the World Health Organization classification system, the major diagnostic criterion for bone marrow involvement by Systemic Mastocytosis (SM) is the presence of dense aggregates (>15 cells) of mast cells. Expression of CD25, a low-affinity receptor for interleukin-2 (IL-2), is a reliable diagnostic tool for distinguishing neoplastic mast cell aggregates from reactive proliferations, and has therefore recently become a minor criterion for the diagnosis of SM. Hahn et al. demonstrated that aberrant staining of Mast cell clusters by anti-CD25 antibody in GI biopsies was essentially diagnostic of SM. Anti-CD25 antibody has also been useful in identifying mast cells in skin biopsies in the setting of Urticaria Pigmentosa, which is predictive of Systemic Mastocytosis. Quantitation of regulatory T cells (Treg) in the setting of hepatocellular carcinoma has been used as an independent predictive factor of tumor recurrence after hepatic resection for HCC. Also, the percentage of tumor-infiltrating CD25+FOXP3+ regulatory T cells among tumor cells, inside tumor parenchyma and at its periphery, is significantly higher in recurrent cutaneous melanoma than in non-recurrent melanoma.

Quality


IVD

IVD

IVD

RUO

Linkage

CD25 Positive Control Slides, Product No. 125S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Preparation Note

Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.

Other Notes

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Legal Information

Cell Marque is a trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Clelia Miracco et al.
Oncology reports, 18(5), 1115-1122 (2007-10-05)
Tumour-infiltrating lymphocytes (TILs) represent the local immune response to cancer, however, their correlation with tumour behaviour is not unanimously considered in the literature. Most studies have not characterized TILs, that are known to comprise distinct subsets, bearing different roles in
Sameer A Siddiqui et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 13(7), 2075-2081 (2007-04-04)
Regulatory T cells (Tregs) have been implicated as inhibitors of antitumoral immunity, and evidence suggests that elimination of Tregs may augment natural and pharmacologic immunity. We tested for the presence of putative Tregs within renal cell carcinoma (RCC) tumors. We
Travis J Hollmann et al.
The American journal of surgical pathology, 32(1), 139-145 (2007-12-29)
Urticaria pigmentosa (UP) is a clinicopathologic term used to describe reddish-brown cutaneous macules and papules, characterized histologically by mast cell infiltration of the papillary and upper reticular dermis and reactive basal hyperpigmentation of the overlying epidermis. Although typically a benign
Hejin P Hahn et al.
The American journal of surgical pathology, 31(11), 1669-1676 (2007-12-07)
Systemic mastocytosis (SM) is characterized by the accumulation of neoplastic mast cells in bone marrow and other organs. Gastrointestinal (GI) symptoms are common in both SM and cutaneous mastocytosis [urticaria pigmentosa (UP)], and are usually caused by the release of

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