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19123

Sigma-Aldrich

Nile Red

BioReagent, suitable for fluorescence, ≥97.0% (HPLC)

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Synonym(s):
Nile Blue A Oxazone
Empirical Formula (Hill Notation):
C20H18N2O2
CAS Number:
Molecular Weight:
318.37
Beilstein/REAXYS Number:
279110
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

product line

BioReagent

Quality Level

assay

≥97.0% (HPLC)

form

solid

mp

203-205 °C (lit.)
205-215 °C

λmax

553 nm

fluorescence

λex 530 nm; λem 635 nm in methanol

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

CCN(CC)c1ccc2N=C3C(Oc2c1)=CC(=O)c4ccccc34

InChI

1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3

InChI key

VOFUROIFQGPCGE-UHFFFAOYSA-N

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1 of 4

This Item
N3013P475872485
Nile Red BioReagent, suitable for fluorescence, ≥97.0% (HPLC)

19123

Nile Red

Essential+ Grade
Nile Red Technical grade

N3013

Nile Red

-
Phenol Red sodium salt pH indicator dye

P4758

Phenol Red sodium salt

-
Nile Red for microscopy

72485

Nile Red

-
fluorescence

λex 530 nm; λem 635 nm in methanol

fluorescence

-

fluorescence

-

fluorescence

-

form

solid

form

powder

form

powder

form

crystals

λmax

553 nm

λmax

553 nm

λmax

-

λmax

553 nm

storage temp.

2-8°C

storage temp.

room temp

storage temp.

room temp

storage temp.

-

assay

≥97.0% (HPLC)

assay

-

assay

-

assay

-

General description

Nile Red is an uncharged hydrophobic molecule and a benzophenoxazone dye. It functions as a fluorescent probe for intracellular lipids and hydrophobic domains of proteins. This dye is fluorescent in all organic solvents. Its fluorescence colors range from golden yellow to deep red.

Application

Nile Red has been used for the staining of intracellular lipid droplets and neutral lipids.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Nile red as a polarity-sensitive fluorescent probe of hydrophobic protein surfaces
Sackett D L and Wolff J
Analytical Biochemistry, 167(2), 228-234 (1987)
Resveratrol suppresses T0901317-induced hepatic fat accumulation in mice
Gao M and Liu D
The AAPS Journal, 15, 744-752 (2013)
Three-dimensional label-free imaging and quantification of lipid droplets in live hepatocytes
Kim K, et al.
Scientific Reports, 6, 36815-36815 (2016)
Development of an in vitro human liver system for interrogating nonalcoholic steatohepatitis
Feaver RE, et al.
JCI insight, 1(20) (2016)
Biosynthesis of high yield fatty acids from Chlorella vulgaris NIES-227 under nitrogen starvation stress during heterotrophic cultivation
Shen XF, et al.
Water Research, 81(3), 294-300 (2015)

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