72485

Nile Red

for microscopy

• Synonym(s): Nile Blue A Oxazone
• Empirical Formula (Hill Notation): C₂₀H₁₈N₂O₂
• CAS Number: 7385-67-3
• Molecular Weight: 318.37
• Beilstein/REAXYS Number: 279110
• EC Number: 230-966-0
• MDL number: MFCD00011639
• UNSPSC Code: 12171500
• PubChem Substance ID: 57652419
• NACRES: NA.32

Properties

• grade: for microscopy
• form: crystals
• mp: 203-205 °C (lit.)
• λmax: 553 nm
• SMILES string: CCN(CC)c1ccc2N=C3C(Oc2c1)=CC(=O)c4ccccc34
• InChI: 1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3
• InChI key: VOFUROIFQGPCGE-UHFFFAOYSA-N

Description

General description

Nile Red is a phenoxazone dye.[1] It is an important stain, which is used to detect intracellular lipid droplets[2] and proteins.[3] Nile Red detects lysosomes and lysosome-related organelles, like gut granules in C. elegans intestinal cells.[4] It exhibits strong fluorescence enhancement and is used for staining SDS (sodium dodecyl sulphate) gels.[1]

Application

Nile Red has been used:

• as a fluorescent dye for staining fat by confocal scanning laser microscopy (CSLM)[5][6][7]
• to label nanoparticles (NPs)[8]
• for lipid analysis[9]

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)