MilliporeSigma
All Photos(1)

Documents

72485

Sigma-Aldrich

Nile Red

for microscopy

Sign Into View Organizational & Contract Pricing

Synonym(s):
Nile Blue A Oxazone
Empirical Formula (Hill Notation):
C20H18N2O2
CAS Number:
Molecular Weight:
318.37
Beilstein:
279110
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.32

grade

for microscopy

form

crystals

mp

203-205 °C (lit.)

λmax

553 nm

SMILES string

CCN(CC)c1ccc2N=C3C(Oc2c1)=CC(=O)c4ccccc34

InChI

1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3

InChI key

VOFUROIFQGPCGE-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
N301319123N5632
Nile Red for microscopy

Sigma-Aldrich

72485

Nile Red

-
Nile Red Technical grade

Sigma-Aldrich

N3013

Nile Red

-
Nile Red BioReagent, suitable for fluorescence, ≥97.0% (HPLC)

Sigma-Aldrich

19123

Nile Red

Essential+ Grade
Nile Blue A Dye content ≥75 %

Sigma-Aldrich

N5632

Nile Blue A

-
form

crystals

form

powder

form

solid

form

powder

mp

203-205 °C (lit.)

mp

203-205 °C (lit.)

mp

203-205 °C (lit.), 205-215 °C

mp

>300 °C (dec.) (lit.)

λmax

553 nm

λmax

553 nm

λmax

553 nm

λmax

-

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100

General description

Nile Red is a phenoxazone dye. It is an important stain, which is used to detect intracellular lipid droplets and proteins. Nile Red detects lysosomes and lysosome-related organelles, like gut granules in C. elegans intestinal cells. It exhibits strong fluorescence enhancement and is used for staining SDS (sodium dodecyl sulphate) gels.

Application

Nile Red has been used:
  • as a fluorescent dye for staining fat by confocal scanning laser microscopy (CSLM)
  • to label nanoparticles (NPs)
  • for lipid analysis

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 5

1 of 5

Fast Green FCF Dye content ≥85 %

Sigma-Aldrich

F7252

Fast Green FCF

Oil Red O certified by the Biological Stain Commission

Sigma-Aldrich

O0625

Oil Red O

Sigma-Aldrich

Sigma-Aldrich

18909

Calcofluor White Stain

Sudan Black B certified by the Biological Stain Commission

Sigma-Aldrich

199664

Sudan Black B

Oil Red O solution 0.5% in isopropanol

Sigma-Aldrich

O1391

Oil Red O solution

Importance of intrinsic properties of dense caseinate dispersions for structure formation
Manski JM, et al.
Biomacromolecules, 8(11), 3540-3547 (2007)
Influence of process parameters on formation of fibrous materials from dense calcium caseinate dispersions and fat
Manski JM, et al.
Food Hydrocolloids, 22(4), 587-600 (2008)
Labeling nanoparticles: Dye leakage and altered cellular uptake
Snipstad S, et al.
Cytometry. Part A : the Journal of the International Society For Analytical Cytology, 91(8), 760-766 (2017)
Influence of shear during enzymatic gelation of caseinate-water and caseinate-water-fat systems
Manski JM, et al.
Journal of Food Engineering, 79(2), 706-717 (2007)
Curcumin/Turmeric as an Environment-friendly Stain for Proteins on Acrylamide Gels
Polyphenols in Human Health and Disease, 779-784 (2014)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service