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20966

Sigma-Aldrich

Cesium chloride

BioUltra, for molecular biology, ≥99.5% (AT)

Synonym(s):

CsCl, cesium monochloride

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About This Item

Linear Formula:
CsCl
CAS Number:
7647-17-8
Molecular Weight:
168.36
EC Number:
231-600-2
MDL number:
MFCD00010955
UNSPSC Code:
12352302
PubChem Substance ID:
57647887
NACRES:
NA.25
assay:
≥99.5% (AT)
grade:
for molecular biology
form:
solid
solubility:
H2O: 3 M at 20 °C, clear, colorless

grade

for molecular biology

Quality Level

200

product line

BioUltra

assay

≥99.5% (AT)

form

solid

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected
≤0.001% total nitrogen (N)

loss

≤0.1% loss on drying, 110 °C

pH

6.0-7.5 (25 °C, 3 M in H2O)

mp

645 °C (lit.)

solubility

H2O: 3 M at 20 °C, clear, colorless

anion traces

sulfate (SO42-): ≤20 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤50 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

3 M in H2O

UV absorption

λ: 260 nm Amax: 0.02
λ: 280 nm Amax: 0.02

SMILES string

[Cl-].[Cs+]

InChI

1S/ClH.Cs/h1H;/q;+1/p-1

InChI key

AIYUHDOJVYHVIT-UHFFFAOYSA-M

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Biochem/physiol Actions

Cesium chloride (CsCl) can be used for the preparation of electrically conducting glasses. It can also be used to make solutions for the separation of RNA from DNA by density gradient centrifugation.

Other Notes

Cesium chloride gradient centrifugation for phage purification; Isolation of satellite DNA; RNA purification; Buoyant density of DNA - Hoechst 33258 complexes in CsCl gradients.

pictograms

Health hazard
GHS08

signalword

Warning

hcodes

H361f

Hazard Classifications

Repr. 2

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCH6572
BCCH3502
BCCG7148
BCCG0925
BCCC9578

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P Karlovsky et al.
Analytical biochemistry, 194(1), 192-197 (1991-04-01)
Buoyant density of DNA in CsCl gradients with Hoechst 33258 (bisbenzimide) was investigated as a function of guanine plus cytosine content of the DNA (%GC; in mole percent). A formula for calculating %GC from the refractive index (nD) of the
Turpen, T. H. and Griffith, O. M.
Biotechnology, 4, 11-11 (1986)
Cooney, C. A. et al.
Methods in Molecular Biology, 2, 21-21 (1984)
Miller, H.
Methods in Enzymology, 152, 159-159 (1987)
Borja Muñoz-Solano et al.
Toxins, 12(6) (2020-06-11)
Mycotoxins are toxic compounds for humans and animals that are produced by fungi. Mycotoxin contamination in feed is a global safety concern and effective control of these compounds in this matrix is needed. This study proposes a simple, cost-effective analytical
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