Skip to Content
MilliporeSigma
All Photos(2)

Documents

27813

Sigma-Aldrich

Coomassie protein assay reagent

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

MDL number:
MFCD00163371
UNSPSC Code:
12171500
PubChem Substance ID:
57648233
NACRES:
NA.21

form

liquid

density

~1.31 g/mL at 20 °C

storage temp.

2-8°C

SMILES string

[Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3C)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5/C=CC(\C=C5C)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1

InChI

1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30H,6-8,31-32H2,1-5H3,(H2,51,52,53,54,55,56);/q;+1/p-1

InChI key

RWVGQQGBQSJDQV-UHFFFAOYSA-M

Related Categories

General description

Coomassie protein assay reagent is a protein analysis reagent used for the determination of total protein concentration. The Coomassie dye in the Coomassie (Bradford) reagent binds to protein in the sample leading to an immediate shift in absorption with a concomitant color change of the solution from red to blue. This spectrophotometric measurement of the intensity of this resulting blue color is used to determine the concentration of protein in solutions.

Application

Suitable for the determination of protein according to Bradford. Five-fold concentrate for tests in microtiter plates; ten-fold concentrate for tests in photometric cuvettes (d=1cm); dilute with H2O

Legal Information

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - STOT SE 1

target_organs

Eyes,Central nervous system

Storage Class

3 - Flammable liquids

wgk_germany

WGK 2

flash_point_f

109.4 °F

flash_point_c

43 °C

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Diwakar Dahal et al.
Plant molecular biology, 73(6), 643-658 (2010-05-25)
Proteomics approach was used to elucidate the molecular interactions taking place at the stem cell wall level when tomato species were inoculated with Ralstonia solanacearum, a causative agent of bacterial wilt. Cell wall proteins from both resistant and susceptible plants
An-Cheng Huang et al.
Oncology letters, 19(3), 2397-2403 (2020-03-21)
Furoquinolone and its derivatives exhibit antimicrobial, anti-allergic, anti-inflammatory and anticancer properties. The present study investigated the anti-tumor activity of synthesized intermediates of furoquinolone in human promyelocytic leukemia HL-60 cells. The biological effects of the active compound ethyl 2-anilino-4-oxo-4,5-dihydrofuran-3-carboxylate (compound 131)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
M M Bradford
Analytical biochemistry, 72, 248-254 (1976-05-07)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service