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B8647

Sigma-Aldrich

Brilliant Blue R Concentrate

suitable for SDS-PAGE, methanol solution

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Synonym(s):
Brilliant Blue R, Acid Blue 83, Brilliant indocyanin 6B, Coomassie Brilliant Blue R
Empirical Formula (Hill Notation):
C45H44N3NaO7S2
CAS Number:
Molecular Weight:
825.97
Colour Index Number:
42660
Beilstein/REAXYS Number:
5718025
MDL number:
PubChem Substance ID:
NACRES:
NA.47

form

methanol solution

Quality Level

suitability

suitable for SDS-PAGE

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=C/C(C=C5)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1

InChI

1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53,54);/q;+1/p-1

InChI key

NKLPQNGYXWVELD-UHFFFAOYSA-M

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This Item
B6529B014927816
Brilliant Blue R Concentrate suitable for SDS-PAGE, methanol solution

B8647

Brilliant Blue R Concentrate

Brilliant Blue R Staining Solution suitable for (for immunoelectrophoresis protein staining)

B6529

Brilliant Blue R Staining Solution

Brilliant Blue R Dye content ~50 %, Technical grade

B0149

Brilliant Blue R

Brilliant Blue R 250, for microscopy

27816

Brilliant Blue R

form

methanol solution

form

ethanol solution

form

powder

form

powder

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Application

Brilliant Blue R concentrate is a methanol based stain especially designed for staining protein in polyacrylamide but will also stain agarose gels. The stain contains methanol and acetic acid so gels do not require fixing prior to staining.
Brilliant Blue R Concentrate has been used for protein band detection after nondenaturing gradient gel electrophoresis, isoelectric focussing and SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis).
For detection of protein bands following electrophoresis.

Reconstitution

After dilution with deionized water to a final volume of one liter, solution will contain 0.25% (w/v) Brilliant Blue R, 40% (v/v) methanol and 7% (v/v) acetic acid.

Legal Information

related product

Product No.
Description
Pricing

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 1

target_organs

Eyes

Storage Class

3 - Flammable liquids

wgk_germany

WGK 3

flash_point_f

57.2 °F - closed cup

flash_point_c

14 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


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Overexpression of human apolipoprotein AI in transgenic rats and the hyperlipoproteinemia associated with experimental nephrosis.
Burkey BF, et al.
Journal of Lipid Research, 36.7, 1463-1473 (1995)
The effect of ethanol or hepatotoxin exposure on rat transferrin desialylation.
Lof K, et al.
Alcohol and Alcoholism (Oxford, Oxfordshire), 31, 445-451 (1996)
Crystallization of soluble proteins in vapor diffusion for x-ray crystallography.
Benvenuti M and Mangani S
Nature Protocols, 2, 1633-1651 (2007)
Katharina Stock et al.
Cancer cell international, 19, 77-77 (2019-04-13)
The nucleation-promoting factor cortactin is expressed and promotes tumor progression and metastasis in various cancers. However, little is known about the biological role of cortactin in the progression of pancreatic ductal adenocarcinoma (PDAC). Cortactin and phosphorylated cortactin (Y421) were investigated
E Zeindl-Eberhart et al.
Electrophoresis, 18(5), 799-801 (1997-05-01)
A new, easy method for the immunodetection of specific antigens in two-dimensional electrophoresis (2-DE) is described. Areas of 2-DE gels containing antigens of interest are electrophoretically transferred to polyvinylidene difluoride membranes, immunostained with specific antibodies using Fast Red or 5-bromo-4-chloro-3-indolyl

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