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C3306

Sigma-Aldrich

Calcium chloride dihydrate

≥99.0%

Synonym(s):

CaCl2.2H2O, Calcium chloride, Calcium dichloride

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About This Item

Empirical Formula (Hill Notation):
CaCl2 · 2H2O
CAS Number:
10035-04-8
Molecular Weight:
147.01
EC Number:
233-140-8
MDL number:
MFCD00149613
UNSPSC Code:
12352302
PubChem Substance ID:
24892603
NACRES:
NA.31
assay:
≥99.0%
grade:
Molecular Biology
form:
powder
solubility:
H2O: 294 mg/mL, clear, colorless
Pricing and availability is not currently available.

grade

Molecular Biology

Quality Level

200

assay

≥99.0%

form

powder

mp

176 °C (dec.) (lit.)

solubility

H2O: 294 mg/mL, clear, colorless

foreign activity

DNase, RNase, protease, none detected

SMILES string

O.O.Cl[Ca]Cl

InChI

1S/Ca.2ClH.2H2O/h;2*1H;2*1H2/q+2;;;;/p-2

InChI key

LLSDKQJKOVVTOJ-UHFFFAOYSA-L

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Application

Calcium chloride dihydrate has been used:
to prepare competent E. coli for transformation(118)
in the preparation of calcium polyphosphate nanospheres and to induce alkaline phosphatase(119)
as a component of renaturation buffer for the zymogram of Staphylococcus aureus crude enzyme extracts(120)

Biochem/physiol Actions

Calcium chloride is used in the heat-shock transformation of microbes with the plasmid. The positive charged calcium forms a cation bridge with phosphate of DNA and negative charged phosphorylated lipid A in lipopolysaccharide (LPS) aiding the DNA transformation into the microbes.[1]

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000446265
0000446265
0000444026
0000444025
0000444024

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Preparation of calcium competent Escherichia coli and heat-shock transformation
Chang AY, et al.
Journal of Experimental Microbiology and Immunology, 1, 22-25 (2017)
Hui Sun Leong et al.
Nature communications, 5, 3947-3947 (2014-05-24)
Non-coding RNAs (ncRNAs) are frequent and prevalent across the taxa. Although individual non-coding loci have been assigned a function, most are uncharacterized. Their global biological significance is unproven and remains controversial. Here we investigate the role played by ncRNAs in
Bradley T Estes et al.
Nature protocols, 5(7), 1294-1311 (2010-07-03)
The ability to isolate, expand and differentiate adult stem cells into a chondrogenic lineage is an important step in the development of tissue engineering approaches for cartilage repair or regeneration for the treatment of joint injury or osteoarthritis, as well
Zhijian Zhao et al.
Scientific reports, 10(1), 4399-4399 (2020-03-12)
The retina decomposes visual stimuli into parallel channels that encode different features of the visual environment. Central to this computation is the synaptic processing in a dense layer of neuropil, the so-called inner plexiform layer (IPL). Here, different types of
Denisa Hathazi et al.
The EMBO journal, 39(23), e105364-e105364 (2020-11-01)
Reversible infantile respiratory chain deficiency (RIRCD) is a rare mitochondrial myopathy leading to severe metabolic disturbances in infants, which recover spontaneously after 6-months of age. RIRCD is associated with the homoplasmic m.14674T>C mitochondrial DNA mutation; however, only ~ 1/100 carriers develop
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