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Human Osteoblasts: HOb, fetal

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Synonym(s):
Hob cells
NACRES:
NA.81

biological source

human bone (normal)

Quality Level

packaging

pkg of 500,000 cells

manufacturer/tradename

Cell Applications, Inc

growth mode

Adherent

karyotype

2n = 46

morphology

osteoblast

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

arthritis; osteoporosis

shipped in

dry ice

storage temp.

−196°C

Related Categories

General description

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HOb are isolated from fetal or adult human bones. Additionally, osteoblasts from osteoarthritis or rheumatoid arthritis donors are available. Human osteoblasts provide an excellent model system for studies relevant to the skeletal system.

Characterization: Positive for bone mineralization.

Human osteoblasts have been used to:
  • Characterize signaling pathways mediating bone morphogenesis, such as identify human Crossveinless-2 as an inhibitor of BMP (Binnerts, 2009); demonstrate crosstalk between Runx2, Osterix, and NELL-1 (Chen, 2011); identify TWEAK as a cytokine regulating RANTES production, BMP-2-induced differentiation, and RANKL expression in osteoblasts (Ando, 2006)
  • Demonstrate that Kobophenol A enhances proliferation of osteoblasts via activation of the p38 pathway (Kwak, 2013) and clarify the relationship between hypertension and osteoporosis by demonstrating that Ang II activates osteoclasts via RANKL induction (Shimizu, 2008, 2012)
  • Identify adiponectin as an activator of IL-6, IL-8, VEGF and MMPs in endothelial cells and osteoblasts, implicating it in the development of arthritis (Lee, 2014)
  • Show that inhibition of NF-κB prevented the progression of bone loss in periodontitis and promoted the wound healing in bone defects through the inhibition of osteoclasts (Shimizu, 2009)
  • Study active compounds from Uraria crinita for their potential to stimulate bone formation and regeneration (Mao, 2014)
  • Investigate properties of osteosarcoma, including the role of fluid pressure in angiogenesis (Nathan, 2008) and novel oncogenes (Both, 2012); and develop better treatment strategies (Ma, 2011)
  • Develop optimal materials, coatings and drug delivery techniques for orthopedic implants and tissue engineering (Frandsen, 2014; Ni, 2012; Pilia, 2013a, b, 2014; Shiels, 2012; Valente, 2012; Zhang, 2010, 2011)

Cell Line Origin

Bone

Application

model for skeletal system research, signaling pathways, bone morphogenesis, gene expression, cell activation and proliferation, wound healing, bone formation and regeneration, treatment strategies, tissue engineering, in vitro bone models, mineralization, hormone treatments

Components

Basal Medium containing 10% FBS & 10% DMSO

Preparation Note

  • 3rd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
  • Can be cultured at least 10 doublings

Subculture Routine

Please refer to the HOb Culture Protocol.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Articles

Primary human osteoblasts and media for the optimal growth and mineralization of human osteoblast cultures. Protocols for handling, cell passaging, media, and product use.

Protocols

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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