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63535

Sigma-Aldrich

Manganese(II) chloride tetrahydrate

BioUltra, Molecular Biology, ≥99.0% (KT)

Synonym(s):

Manganese chloride tetrahydrate, Manganese dichloride tetrahydrate, MnCl2.4H2O

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About This Item

Linear Formula:
MnCl2 · 4H2O
CAS Number:
13446-34-9
Molecular Weight:
197.91
EC Number:
231-869-6
MDL number:
MFCD00149792
UNSPSC Code:
12352302
PubChem Substance ID:
57651943
NACRES:
NA.25
assay:
≥99.0% (KT)
grade:
Molecular Biology
solubility:
H2O: 0.5 M at 20 °C, clear, colorless
Pricing and availability is not currently available.

grade

Molecular Biology

Quality Level

200

product line

BioUltra

assay

≥99.0% (KT)

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

pH

4-6 (25 °C, 0.5 M in H2O)

mp

58 °C (lit.)

solubility

H2O: 0.5 M at 20 °C, clear, colorless

anion traces

sulfate (SO42-): ≤50 mg/kg

λ

0.5 M in H2O

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Other Notes

In the tailing of DNA - MnCl2 buffer recommended for dC and dG tailing[1]; In reverse transcription of RNA with thermostable DNA polymerase[2]

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Eye Dam. 1 - STOT RE 2

target_organs

Brain

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk_germany

WGK 2

flash_point_f

does not flash

flash_point_c

does not flash

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM5039
BCCL6420
BCCH2663
BCCJ4597
BCCD2337

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T W Myers et al.
Biochemistry, 30(31), 7661-7666 (1991-08-06)
A recombinant DNA polymerase derived from the thermophilic eubacterium Thermus thermophilus (Tth pol) was found to possess very efficient reverse transcriptase (RT) activity in the presence of MnCl2. Many of the problems typically associated with the high degree of secondary
Nicholas Sofos et al.
Molecular cell, 79(5), 741-757 (2020-07-31)
Cmr-β is a type III-B CRISPR-Cas complex that, upon target RNA recognition, unleashes a multifaceted immune response against invading genetic elements, including single-stranded DNA (ssDNA) cleavage, cyclic oligoadenylate synthesis, and also a unique UA-specific single-stranded RNA (ssRNA) hydrolysis by the
Terminal transferase: use of the tailing of DNA and for in vitro mutagenesis.
G Deng et al.
Methods in enzymology, 100, 96-116 (1983-01-01)
Lisha Luo et al.
NMR in biomedicine, 25(12), 1360-1368 (2012-05-11)
The aim of this study was to provide data on the dose dependence of manganese-enhanced MRI (MEMRI) in the visual pathway of experimental rats and to study the toxicity of MnCl₂ to the retina. Sprague-Dawley rats were intravitreally injected with
Oliviero L Gobbo et al.
Contrast media & molecular imaging, 7(6), 542-546 (2012-09-20)
Lung parenchyma remains one of the most difficult tissues to be imaged by means of magnetic resonance imaging (MRI). Several MRI techniques are routinely used for lung imaging. However, manganese-enhancement MRI (MEMRI) technique has not been associated with pulmonary MRI.
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