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82452

Sigma-Aldrich

Proteinase K, immobilized on Eupergit® C from Tritirachium album

powder (granular), ≥1500 U/g

Synonym(s):

Endopeptidase K, immobilized on Eupergit® C

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.54

biological source

fungus (Tritirachium album)

form

powder (granular)

specific activity

≥1500 U/g

storage temp.

2-8°C

General description

Proteinase K is secreted extracellular medium by mold, Tritirachium album. It hydrolyses keratin and shows substrate specificity similar to serine alkaline proteinases.

Application

Proteinase K, immobilized on Eupergit® C from Tritirachium album has been used:
  • in the protolysis of human serum
  • in the digestion of gruel samples for enzyme linked immune assays (ELISA)
  • in proteolytic stability studies of calcium ion flux inducers

Biochem/physiol Actions

Proteinase K hydrolyses keratin by cleaving peptide bonds adjacent to the carboxyl group of aliphatic and aromatic amino acids.

Unit Definition

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol N-acetyl-L-tyrosine-ethylester (ATEE) per minute at pH 9.0 and 30 °C.

Legal Information

Eupergit is a registered trademark of Röhm GmbH & Co. KG

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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