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Sigma-Aldrich

Protein A soluble from Staphylococcus aureus (Cowan strain)

powder, white

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MDL number:
NACRES:
NA.46

biological source

Staphylococcus aureus

Quality Level

conjugate

unconjugated

form

powder

species reactivity

mouse, cat, monkey, pig, dog, human, rabbit, guinea pig

technique(s)

ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

color

white

capacity

~10 mg/mg binding capacity (human IgG)

solubility

H2O: 1 mg/mL, clear, colorless

UniProt accession no.

storage temp.

2-8°C

Gene Information

Staphylococcus aureus subsp. aureus NCTC 8325 ... SAOUHSC_00069(3919448)

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This Item
P3838P6031H9395
biological source

Staphylococcus aureus

biological source

-

biological source

Staphylococcus aureus

biological source

Staphylococcus aureus

form

powder

form

essentially salt-free, lyophilized powder

form

essentially salt-free, lyophilized powder

form

lyophilized powder

solubility

H2O: 1 mg/mL, clear, colorless

solubility

-

solubility

H2O: soluble 1 mg/mL, clear, colorless

solubility

H2O: soluble 0.49-0.51 mg/mL

UniProt accession no.

P02976

UniProt accession no.

P02976

UniProt accession no.

P02976

UniProt accession no.

Q2G1X0

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

Protein A is a highly stable cell surface receptor produced by several strains of Staphylococcus aureus. This protein is capable of binding to the Fc portion of immunoglobulins, especially IgGs, from a large number of species. One protein A molecule has been shown to bind at least 2 molecules of IgG simultaneously. The IgG binding domain of Protein A consists of three anti-parallel alpha-helicies, the third of which is disrupted when the protein is complexed with the Fc region of the immunoglobulins. Protein A will bind the Fc portion of human IgG subclasses, IgM, IgA and IgE; and mouse IgG1 (weakly), IgG2a, and IgG2b. Protein A also associates with IgGs from other species, including monkey, rabbit, pig, guinea pig, dog, and cat.
Protein A is involved in regulating anti-tumor, toxic, and carcinogenic functions. In addition to acting as an immunomodulator, it also has antifungal and antiparasitic properties.

Application

Protein A may be conjugated with various reporter molecules such as fluorescent dyes (FITC), enzyme markers (peroxidase, beta-galactosidase, alkaline phosphatase), biotin, and colloidal gold without affecting the antibody binding site on the molecule. These conjugates may be used to detect immunoglobulins using immunochemical assays such as western blotting, immunohistochemistry, and ELISA. In addition, protein A may also be immobilized on a solid supports like agarose or acrylic beads for the purification of polyclonal or monoclonal immunoglobulins. It is also routinely used for immunoprecipitation assays. Furthermore, protein A has been used for affinity chromatography and SDS-PAGE assays.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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