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L8777

Sigma-Aldrich

Lectin from Phytolacca americana (pokeweed)

lyophilized powder, BioReagent, suitable for cell culture

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Synonym(s):
PWM, Pokeweed mitogen
MDL number:
NACRES:
NA.32

sterility

aseptically filled

Quality Level

product line

BioReagent

form

lyophilized powder

potency

≤0.3 μg/mL mitogenic activity

technique(s)

cell culture | mammalian: suitable

solubility

PBS: 1 mg/mL

storage temp.

2-8°C

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This Item
L414461764L2895
Sigma-Aldrich

61764

Lectin from Phaseolus vulgaris

form

lyophilized powder

form

lyophilized powder

form

powder

form

lyophilized powder

solubility

PBS: 1 mg/mL

solubility

DPBS: 5 mg/mL, slightly hazy, colorless

solubility

-

solubility

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

-

Quality Level

200

potency

≤0.3 μg/mL mitogenic activity

potency

erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at 250 μg lectin/ml.), ≤1.0 μg per mL leucoagglutination activity, ≤5 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells)

potency

-

potency

<50 μg per mL agglutination activity (using human blood group B erythrocytes)

General description

Lectins are proteins of plant origin, which have the ability to bind to certain neuronal surface glycoproteins and glycolipids. This ability of theirs helps them to be used as neuroanatomical tracers, as they are taken and transported in a retrograde or anterograde fashion.

Application

Lectin from Phytolacca americana (pokeweed) is used for proliferation Assay. (Cellular proliferative responses were measured by the incorporation of 3H thymidine in newly synthesized DNA, using a conventional assay).

Biochem/physiol Actions

Lectins are carbohydrate-binding proteins and are highly specific for sugar moieties. Pokeweed lectin (PL) is a lectin specific for N-acetylglucosamine-containing saccharides that stimulate peripheral lymphocytes to undergo mitosis by binding to their cell surfaces. Four types of lectins are isolated from the roots of pokeweed (Phytolacca americana). They contain homologous domains but have different molecular sizes and biological properties.
PWM is extracted from pokeweed roots. This protein has hemagglutinating, leukoagglutinating, and mitogenic properties. PWM is not blood group specific, but has affinity for N-acetyl-β-D-glucosamine oligomers.

Quality

Partially purified TCA precipitate.
Essentially salt-free.

Analysis Note

Mitogenic activity was determined by BrdU incorporation in HeLa cell cultures.

pictograms

Health hazard

signalword

Danger

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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Kurt Hanevik et al.
The Journal of infectious diseases, 204(11), 1779-1786 (2011-10-13)
Clinical and epidemiological studies have suggested the development of acquired immunity in individuals previously infected with Giardia lamblia. However, there are no data on the long-term cellular immunity and genotype cross-reactivity. An outbreak of assemblage B giardiasis in a nonendemic
Caroline Leeth et al.
Animals : an open access journal from MDPI, 11(10) (2021-10-24)
Immunological studies in the horse are frequently hampered by lack of environmental control, complicated study design, and ethical concerns when performing high risk studies. The purpose of the current study was to investigate the utility of a xenograft model for
Tomomi Fujii et al.
Acta crystallographica. Section D, Biological crystallography, 60(Pt 4), 665-673 (2004-03-25)
Pokeweed lectin (PL), a lectin specific for N-acetylglucosamine-containing saccharides, stimulates peripheral lymphocytes to undergo mitosis by binding to their cell surfaces. Four types of lectins have been isolated from the roots of pokeweed (Phytolacca americana) and shown to contain homologous
Sharon N Cox et al.
Kidney international, 78(4), 396-407 (2010-05-21)
Immunoglobulin A nephropathy (IgAN) is the most common form of primary glomerulonephritis worldwide. The basic defect lies within the IgA immune system and in peripheral blood leukocytes, rather than local kidney abnormalities. To define the intracellular mechanisms leading to the
A randomized, placebo-controlled phase I study assessing the safety and immunogenicity of a Pseudomonas aeruginosa hybrid outer membrane protein OprF/I vaccine (IC43) in healthy volunteers.
Westritschnig K, Hochreiter R, Wallner G, et al.
Human Vaccines & Immunotherapeutics, 10(1), 170-183 (2014)

Articles

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