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L1668

Sigma-Aldrich

Lectin from Phaseolus vulgaris (red kidney bean)

Phytohemagglutinin PHA-P, lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl), BioReagent, suitable for cell culture

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Synonym(s):
Phaseolus vulgaris agglutinin, PHA
NACRES:
NA.32

Quality Level

product line

BioReagent

form

lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl)

potency

≤10 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells)

technique(s)

cell culture | mammalian: suitable

color

white to off-white

solubility

PBS: 5 mg/mL, clear to slightly hazy, colorless to faintly yellow (pH 6.8)

storage temp.

2-8°C

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This Item
L8902L8754L8629
solubility

PBS: 5 mg/mL, clear to slightly hazy, colorless to faintly yellow (pH 6.8)

solubility

PBS: 2 mg/mL

solubility

-

solubility

-

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

300

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

potency

≤10 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells)

potency

<40 μg per mL agglutination activity

potency

≤62.5 μg per mL agglutination activity, ≤10 μg/mL mitogenic activity

potency

<10 μg per mL agglutination activity (with erythryocytes)

form

lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl)

form

lyophilized powder

form

lyophilized powder

form

salt-free, lyophilized powder

Application

Lectin from Phaseolus vulgaris (red kidney bean) has been used:
  • for the stimulation of cluster of differentiation (CD4+) T cells
  • for the activation of primary peripheral blood lymphocytes
  • for the stimulation of peripheral blood mononuclear cells

Biochem/physiol Actions

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Packaging

Sold on the basis of lectin content

Analysis Note

Potency and solubility expressed in mg lectin/ml

related product

Product No.
Description
Pricing

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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