89050907
LLC-RK1 cell line
rabbit kidney, Epithelial-like
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About This Item
UNSPSC Code:
41106514
NACRES:
NA.81
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product name
LLC-RK1 cell line, from rabbit kidney
biological source
rabbit kidney
form
liquid
growth mode
Adherent
karyotype
Modal no. 45 (2n=46)
morphology
Epithelial-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Description
Derived from kidneys taken from New Zealand white rabbits. The cells are susceptible to a range of viruses including rubella, vaccinia and herpes. Rubella replicates to a high titre.
Culture Medium
Medium 199 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 5x10,000 to 3x100,000 cells/cm2using 0.25% trypsin; 5% CO2; 37°C. NB: this line is extremely sensitive to trypsin.
Other Notes
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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DEVELOPMENT AND CHARACTERISTICS OF THE RABBIT KIDNEY CELL STRAIN, LLC-RK.
R N HULL et al.
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.), 118, 1054-1059 (1965-04-01)
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