P9792
Phytase from Aspergillus ficuum
lyophilized powder, 1-5 units/mg solid (as phosphatase activity)
Synonym(s):
3-Phytase, myo-Inositol-hexakisphosphate 3-phosphohydrolase
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
Recommended Products
form
lyophilized powder
specific activity
1-5 units/mg solid (as phosphatase activity)
storage temp.
−20°C
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Quality
May contain glucosidases and phosphatases.
Unit Definition
One unit will liberate 1.0 μmole of inorganic P from 4.2 × 10-2 M Mg·K phytate per min at pH 2.5 at 37 °C.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificates of Analysis (COA)
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Duane F Berry et al.
Bioorganic & medicinal chemistry letters, 15(12), 3157-3161 (2005-05-10)
A novel approach for measuring phytase activity is presented. We have developed a new chromophoric substrate analog of phytic acid, 5-O-[6-(benzoylamino)hexyl]-d-myo-inositol-1,2,3,4,6-pentakisphosphate that permits direct measurement of the phosphate ester bond-cleavage reaction using HPLC. This compound, along with its dephosphorylated T-phosphatidylinositol
Molecular and physiological characterisation of a 3-phytase from soil bacterium Klebsiella sp. ASR1.
A Sajidan et al.
Applied microbiology and biotechnology, 65(1), 110-118 (2004-01-17)
Klebsiella sp. strain ASR1 isolated from an Indonesian rice field is able to hydrolyse myo-inositol hexakis phosphate (phytate). The phytase protein was purified and characterised as a 42 kDa protein accepting phytate, NADP and sugar phosphates as substrates. The corresponding
J Dvoráková et al.
Folia microbiologica, 45(2), 128-132 (2001-03-29)
Kinetics of phytate hydrolysis by Aspergillus niger phytase and correlation between the amount of released phosphate and creation of lower myo-inositol phosphates were investigated. Phytase was able to hydrolyze myo-inositol hexakis-, pentakis-, tetrakis-, and trisphosphates. Finally, about 56% of total
Nada M Tamim et al.
Journal of agricultural and food chemistry, 51(16), 4687-4693 (2004-01-07)
Phytate phosphorus (PP) hydrolysis by a 3-phytase was studied in vitro at pH 2.5 and 6.5 with either 0, 1.0, 4.0, or 9.0 g of Ca/kg diet, or 0, 1.0, 5.0, 7.5, or 10.0 g/kg diet of micro-mineral premix added
R L Payne et al.
Poultry science, 84(2), 265-272 (2005-03-04)
Research with corn-soybean meal diets was conducted to compare phytase sources in commercial broilers. A Ca to nonphytate P (nPP) ratio of 2.5:1 was maintained in all diets. Experiments 1 and 2 were conducted from d 4 to 13 (experiment
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