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A0303

Sigma-Aldrich

Anti-Water Channel Aquaporin 3 antibody produced in rabbit

affinity isolated antibody

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Synonym(s):
anti-AQP3
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat, mouse

technique(s)

immunohistochemistry: suitable using Mouse kidney sections
western blot: 1:200 using Rat kidney membranes

UniProt accession no.

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... Aqp3(11828)
rat ... Aqp3(65133)

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This Item
A5971A5560A7310
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

antibody form

IgG fraction of antiserum

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

species reactivity

rat, mouse

species reactivity

human, mouse, rat

species reactivity

rat, human

species reactivity

rat, human

technique(s)

immunohistochemistry: suitable using Mouse kidney sections, western blot: 1:200 using Rat kidney membranes

technique(s)

flow cytometry: suitable (indirect), immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot (chemiluminescent): 1:400-1:800

technique(s)

immunohistochemistry: suitable, western blot (chemiluminescent): 1:200-1:1000

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General description

Aquaporins function in the movement of water and small solutes across the cell membrane. Aquaporins are widely distributed from bacteria to plants and animals. In E. coli, one aquaporin has been identified that is named AQP-Z. In the plant Arabidopsis thaliana, over 9 AQPs have been characterized. In mammals, at least 11 different AQPs have been cloned. AQP3 provides kidney medullary collecting ducts with high permeability to water, permitting water to move in the direction of an osmotic gradient and may play an important role in gastrointestinal tract water transport. AQP3 and AQP4 are located at the basolateral part of epithelial cells in kidney, colon and trachea.
Rabbit polyclonal anti-Water Channel Aquaporin 3 recognizes rat and mouse aquaporin 3 transport proteins.

Immunogen

synthetic peptide corresponding to amino acids 275-292 of rat aquaporin 3 (with additional N-terminal lysine and tyrosine) conjugated to KLH with glutaraldehyde

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Rabbit anti-AQP3 antibody was used in western blot analysis to identify the level of aquaporin 3 in E. solidaginis larvae. Anti-AQP3 was diluted 1:200 in 5% nonfat dry milk and incubated with the membrane for 2hrs at 21 degrees C.
Rabbit polyclonal anti-Water Channel Aquaporin 3 is used to tag aquaporin 3 for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of aquaporin 3 in kidney medullary collecting ducts and the basolateral surfaces of epithelial cells involved in water transport.

Physical form

Lyophilized from a solution containing phosphate buffered saline, pH 7.4, 1% BSA, and 0.05% sodium azide

Preparation Note

Affinity isolated on immobilized Protein A

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Susumu Yoshie et al.
Journal of cellular physiology, 234(9), 15951-15962 (2019-02-05)
Functional central airway epithelial cells (CAECs) from induced pluripotent stem cells (iPSCs) are an attractive potential cell source for central airway regeneration. The central airway epithelium, such as the tracheal epithelium, is composed of ciliated cells, goblet cells, and basal
M Echevarria et al.
Proceedings of the National Academy of Sciences of the United States of America, 91(23), 10997-11001 (1994-11-08)
The terminal part of the inner medullary collecting duct exhibits a high degree of water permeability that is independent of increased intracellular cAMP and not accounted for by the activity of the known renal epithelial water channels CHIP28 (28-kDa channel-forming
Susumu Yoshie et al.
Journal of cellular physiology (2019-02-05)
Functional central airway epithelial cells (CAECs) from induced pluripotent stem cells (iPSCs) are an attractive potential cell source for central airway regeneration. The central airway epithelium, such as the tracheal epithelium, is composed of ciliated cells, goblet cells, and basal
Joseph O Lamontagne et al.
Nature communications, 13(1), 2226-2226 (2022-04-27)
Transcription factors AP-2α and AP-2β have been suggested to regulate the differentiation of nephron precursor populations towards distal nephron segments. Here, we show that in the adult mammalian kidney AP-2α is found in medullary collecting ducts, whereas AP-2β is found
Tom T Solbu et al.
Chemical senses, 37(1), 35-46 (2011-07-13)
The sense of smell is conveyed by the olfactory sensory neurons of the olfactory mucosa. Uniquely for sensory systems, the olfactory neurons directly face the external environment and are thus vulnerable to infections and changes in the airway surface liquid

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