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A1346

Sigma-Aldrich

ASB-14

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Synonym(s):
3-[N,N-Dimethyl(3-myristoylaminopropyl)ammonio]propanesulfonate, Amidosulfobetaine-14
Empirical Formula (Hill Notation):
C22H46N2O4S
CAS Number:
Molecular Weight:
434.68
MDL number:

description

zwitterionic

assay

≥95.0% (HPLC)

form

powder

mol wt

average mol wt >10,000

aggregation number

≥23

storage temp.

2-8°C

InChI

1S/C22H46N2O4S/c1-4-5-6-7-8-9-10-11-12-13-14-17-22(25)23-18-15-19-24(2,3)20-16-21-29(26,27)28/h4-21H2,1-3H3,(H-,23,25,26,27,28)

InChI key

UTSXERRKRAEDOV-UHFFFAOYSA-N

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This Item
182750SAB1403263SAB1407509
vibrant-m

A1346

ASB-14

vibrant-m

182750

ASB-14

form

powder

form

solid

form

buffered aqueous solution

form

buffered aqueous solution

assay

≥95.0% (HPLC)

assay

≥97% (HPLC)

assay

-

assay

-

mol wt

average mol wt >10,000

mol wt

-

mol wt

antigen ~38.1 kDa

mol wt

antigen ~38.9 kDa

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

100

description

zwitterionic

description

-

description

-

description

-

General description

ASB-14 comprises alkyl tails and amidosulfobetaine headgroup.

Application

ASB-14 has been used as a component of rehydration buffer for proteomic sample preparation from Leishmania donovani.
Zwitterionic detergent. Useful for solubilization of proteins, including membrane proteins, for 2D electrophoresis.

Biochem/physiol Actions

ASB-14 is useful in proteasome extraction especially for the membrane associated proteins. Along with CHAPS (3-(3-cholamidopropyl)dimethylammonio)-1-propanesulfonate), it is employed in protein solubilization even in the presence of thiourea.

Analysis Note

ASB-14 (amidosulfobetaine-14), a membrane solubilizing zwitterionic detergent (medium chain amidosulfobetaine surfactant), may be used in protein and macromolecule research for the preparative extraction and solubilization of proteins for separation and analysis by methods such as 2D-electrophoresis. ASB-14 may be used in tissue engineering for antigen removal (AR) for xenogeneic scaffold generation. ASB-14 may be used to develop and study zwitterionic micelles.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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M P Molloy et al.
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Many bacterial outer membrane proteins (OMPs) are missing from two-dimensional (2-D) gel proteome maps. Recently, we developed a technique for 2-D electrophoresis (2-DE) of Escherichia coli OMPs using alkaline pH incubation for isolation of OMPs, followed by improved solubilization conditions
Thermodynamic and structural characterization of zwitterionic micelles of the membrane protein solubilizing amidosulfobetaine surfactants ASB-14 and ASB-16
D Andrea MG, et al.
Langmuir, 27(13), 8248-8256 (2011)
Francisella t ularensis Proteome: Low Levels of ASB-14 Facilitate the Visualization of Membrane Proteins in Total Protein Extracts
Twine SM, et al.
Journal of Proteome Research, 4(5), 1848-1854 (2005)
Protein Sample Preparation for Proteomic Analysis in Leishmania donovani
Alexandros A and Despina S
Bio-protocol, 4(5), 70-75 (2014)

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