A2074
Monoclonal Anti-Rabbit Immunoglobulins–Peroxidase antibody produced in mouse
clone RG-16, purified immunoglobulin, lyophilized powder
Synonym(s):
Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM)
Sign Into View Organizational & Contract Pricing
All Photos(2)
About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
Recommended Products
biological source
mouse
Quality Level
conjugate
peroxidase conjugate
antibody form
purified immunoglobulin
antibody product type
secondary antibodies
clone
RG-16, monoclonal
form
lyophilized powder
species reactivity
rabbit
should not react with
pig, chicken, bovine, horse, human, guinea pig, turkey, rat, canine, goat, feline, sheep
packaging
vial of 0.5 mL
technique(s)
direct ELISA: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissue
western blot (chemiluminescent): 1:160,000
isotype
IgG1
storage temp.
2-8°C
target post-translational modification
unmodified
Looking for similar products? Visit Product Comparison Guide
Related Categories
General description
Monoclonal Anti-Rabbit Immunoglobulins (mouse IgG1 isotype) is derived from the RG-16 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with rabbit IgG. Immunoglobulins (Igs) belongs to the immunoglobulin super-family. There are five different types of Immunoglobulins. Each immunoglobulin has two heavy (H) and two light (L) chains held together by disulfide linkages. Each light comprises one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region. IgG is abundant immunoglobulin in human serum. The four classes of IgG can be IgG1, IgG2, IgG3, and IgG4.
Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM.
Specificity
Binds only rabbit Igs. Does not react with reduced rabbit Igs.
Application
Monoclonal Anti-Rabbit Immunoglobulins-Peroxidase antibody produced in mouse has been used in:
- western blotting
- immunoblotting
- immunoprecipitation
- enzyme linked immunosorbent assay (ELISA)
- immunohistochemistry
Mouse monoclonal anti-rabbit immunoglobulins have been used for western blot assays at a dilution of 1:1000 using bovine trabecular meshwork cell lysates. The antibody can also be used for immunohistochemistry assays (1:100, using human tissue).
The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature.
Physical form
Lyophilized from 0.01 M sodium phosphate buffer, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT
Preparation Note
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 2
ppe
Eyeshields, Gloves, type N95 (US)
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Pei-Hua Li et al.
Oncology letters, 15(4), 5137-5142 (2018-03-20)
c-Jun activation domain-binding protein-1 (Jab1) is a multifunctional protein involved in cell proliferation and apoptosis, DNA damage and repair and genome stability. In a number of types of human carcinoma, the abnormal expression of Jab1 is associated with poor prognosis
Cheng Chen et al.
European journal of histochemistry : EJH, 65(3) (2021-10-01)
Individuals with diabetes are exposed to a higher risk of perioperative stroke than non-diabetics mainly due to persistent hyperglycemia. LncRNA Meg3 has been considered as an important mediator in regulating ischemic stroke. However, the functional and regulatory roles of Meg3
Nazish Ahmed et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 20(5), 665-678 (2007-09-01)
Chondral defects show lack of proper regeneration whereas osteochondral lesions display limited regeneration capacity. Latter is probably due to immigration of chondroprogenitor cells from the subchondral bone. Known chondroprogenitor cells for cartilage tissues are multi-potent adult marrow stromal or mesenchymal
Martina Redova et al.
International journal of molecular medicine, 25(2), 271-280 (2010-01-01)
We investigated the possible modulation by LOX/ COX inhibitors of all-trans retinoic acid (ATRA)-induced cell differentiation in two established neuroblastoma cell lines, SH-SY5Y and SK-N-BE(2). Caffeic acid, as an inhibitor of 5-lipoxygenase, and celecoxib, as an inhibitor of cyclooxygenase-2, were
Isabelle Gillot et al.
International journal of cell biology, 2009, 524858-524858 (2010-02-11)
During prophase I of male meiosis, the sex chromosomes form a compact structure called XY body that associates with the nuclear membrane of pachytene spermatocytes. Ret Finger Protein is a transcriptional repressor, able to interact with both nuclear matrix-associated proteins
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service