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I0138

Sigma-Aldrich

Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM) antibody produced in mouse

clone RG-16, ascites fluid

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Synonym(s):
Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM)
NACRES:
NA.46

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

secondary antibodies

clone

RG-16, monoclonal

contains

15 mM sodium azide

should not react with

pig, chicken, bovine, horse, human, guinea pig, turkey, rat, canine, goat, feline, sheep

technique(s)

indirect ELISA: 1:10,000

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
R1008I6760A2306
isotype

IgG1

isotype

IgG1

isotype

IgG2a

isotype

IgG1

technique(s)

indirect ELISA: 1:10,000

technique(s)

dot blot: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunohistochemistry (frozen sections): suitable, indirect ELISA: 1:2,000, western blot: suitable

technique(s)

indirect ELISA: 1:3,000

technique(s)

direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160 using human tonsil and Cat. No. I8635 as the primary antibody, western blot: 1:160,000-1:320,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg per well)

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified immunoglobulin

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

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General description

Monoclonal Anti-Rabbit Immunoglobulins (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins are the members of the eponymous immunoglobulin super-family (IgSF). Immunoglobulins are heterodimeric proteins characterized with two heavy (H) and two light (L) chains. Based on heavy chain C domains immunoglobulins are classified in to five main classes namely IgM, IgG, IgA, IgD, and IgE isotypes.
Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM.

Specificity

Binds only rabbit Igs. Does not react with reduced rabbit Igs.

Application

Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM) antibody produced in mouse has been used in enzyme linked immunosorbent assay (ELISA), immunoblots, dot blots and immunohistochemistry.
The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature.

Biochem/physiol Actions

Immunoglobulins acts as a cell-surface receptor for antigen that allows cell signaling and cell activation. In addition, it also acts as a soluble effector molecule that can recognize, bind and neutralize antigens. IgG contributes to immune response including neutralization of toxins and viruses. IgM provides first line of defense and is involved in immunoregulation. IgA protects mucous membranes against toxins, virus and bacteria by direct neutralization or by prevention of binding to the mucosal membranes.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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