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A2105

Sigma-Aldrich

Anti-AP Endonuclease antibody, Mouse monoclonal

clone APEREF, purified from hybridoma cell culture

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Synonym(s):
Anti-AP lysase, Anti-APE/Ref1, Anti-APE1, Anti-APEN, Anti-APEX nuclease, Anti-APEX1, Anti-Apurinic/Apyrimidinic Endonuclease, Anti-HAP1, Monoclonal Anti-AP Endonuclease antibody produced in mouse
MDL number:
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

APEREF, monoclonal

form

buffered aqueous solution

mol wt

antigen ~37 kDa

species reactivity

canine, human, rat, mouse

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using total cell extract of Raji cells

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... APEX1(328)
mouse ... Apex1(11792)
rat ... Apex1(79116)

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A5230SAB4200479SAB1403556
Gene Information

human ... APEX1(328)
mouse ... Apex1(11792)
rat ... Apex1(79116)

Gene Information

human ... ARF6(382)
rat ... Arf6(79121)

Gene Information

human ... SET(6418)
mouse ... Set(56086)
rat ... Set(307947)

Gene Information

human ... APEX1(328)

species reactivity

canine, human, rat, mouse

species reactivity

bovine, canine, rat, human

species reactivity

rat, human, mouse

species reactivity

human

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

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General description

AP endonuclease (APE/Ref1 or Apurinic/apyrimidinic endonuclease) is a crucial enzyme in the DNA base excision repair (BER) pathway. It recognizes baseless sites in the DNA following spontaneous base loss or removal of a damaged base by different DNA glycosylases. This enzyme is the major AP endonuclease in mammalian cells. The human enzyme shows a high degree of homology with other mammalian AP endonucleases (bovine, mouse, rat, and hamster). APE/Ref1 is a multifunctional enzyme. Its N-terminal region includes a nuclear localization sequence and a redox activity zone, while its AP endonuclease activity resides in the C-terminal region. Redox-factor 1 (Ref1) refers to the reduction/oxidation function of the enzyme.
Monoclonal Anti-AP Endonuclease recognizes human, canine, rat, and mouse AP endonuclease (approx. 37kDa).
The AP endonuclease is a DNA damage repair gene involved in base excision repair.

Immunogen

recombinant human AP endonuclease.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Mouse monoclonal anti-AP endonuclease antibodies have been used for western blot analysis. The antibody can also be used for microarray, indirect ELISA and immunocytochemistry assays.

Biochem/physiol Actions

AP endonuclease (APE/Ref1 or Apurinic/apyrimidinic endonuclease) has a 3′-repair diesterase or phosphatase activity. The major DNA repair activity of the enzyme is nicking the DNA phosphodiester backbone 5′ to the AP site leaving a 3′-hydroxyl group and a 5′-deoxyribose phosphate. APE/Ref1 stimulates the DNA binding activity of many transcription factors (Fos, Jun, NF?B, PAX, HIF1, p53 and CREB), which are involved in cancer promotion and progression. The protein was found at elevated levels in several tumors (ovarian, prostate, cervical and germ cells). Redox-factor 1 (Ref1) maintains transcription factors in their active reduced states.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Going APE over ref-1
EvansAR, et al.
Mutation Research, 461(2), 83-108 (2000)
Yong Jun Choi et al.
Genesis (New York, N.Y. : 2000), 49(2), 92-97 (2011-02-24)
Transgenesis enables the elucidation of gene function; however, constant transgene expression is not always desired. The tetracycline responsive system was devised to turn on and off transgene expression at will. It has two components: a doxycycline (dox)-controlled transactivator (TA) and
Xiao Yao et al.
PloS one, 10(10), e0139416-e0139416 (2015-10-09)
We have previously shown that mitochondria-targeted vitamin E (Mito-Vit-E), a mtROS specific antioxidant, improves cardiac performance and attenuates inflammation in a pneumonia-related sepsis model. In this study, we applied the same approaches to decipher the signaling pathway(s) of mtROS-dependent cardiac

Articles

DNA damage and repair mechanism is vital for maintaining DNA integrity. Damage to cellular DNA is involved in mutagenesis, the development of cancer among others.

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